The whole root was divided into crown roots and lateral roots. Each kind of roots was counted manually and measured with a ruler. A scanner (Epson 1680, Indonesia) was used to scan the roots and the scanned images were analyzed using the WinRHIZO software (version 5.0) (Regent Instruments Inc., Quebec City, QC, Canada) to get total root length and average diameter following previously described methods (Peng et al., 2010 (link)). Lateral root density was defined as the number of lateral roots per unit length of crown root with lateral roots. Each treatment had six biological replicates.
Winrhizo software version 5.0
Manufactured by Regent Instruments
Sourced in Canada
WinRHIZO is a software solution developed by Regent Instruments for the analysis of root systems. It is designed to capture, measure, and analyze digital images of root structures. The software provides tools for users to quantify various root characteristics, such as length, surface area, and volume, among other parameters. WinRHIZO is currently available in version 5.0.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using winrhizo software version 5.0
1
Comprehensive Root Morphology Analysis
2
Root Scanning and Analysis Protocol
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The whole root was well spread and scanned with a scanner (Epson 1680, Indonesia). The scanned images were analyzed using the WinRHIZO software (version 5.0) (Regent Instruments Inc., Quebec City, QC, Canada) to get the total root length following the previously described method (Peng et al., 2010 (link)). Each type of root was counted manually. The crown root length was measured with a ruler. The lateral root density was defined as the number of lateral roots per unit of the crown root length.
3
Assessing Root System Architecture
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The root and shoot dry weight was determined after separately drying in the oven at 65°C till constant weight. The root-to-shoot ratio was calculated on a dry matter basis. Each sample had six independent biological replicates, and each replicate was averaged by two samples harvested from the same pot.
At harvest, the entire root system dissected from the root-shoot junction was rinsed three times by DW and stored at –4°C until scan analysis. Each root was placed on a transparent plastic slide and thoroughly combed apart in DW. The desktop scanner (Epson Perfection V850 Pro) was used to scan the roots at a resolution of 400 dpi. The scanned images were analyzed using the WinRHIZO software (version 5.0) (Regent Instruments Inc., Quebec City, QC, Canada) to quantify root indexes as described (Freschet et al., 2020 (link)). The relative root growth rate (RRGR) was computed as a given equation (Samal et al., 2010 (link)).
Where, ln is natural log, TRL is total root length in cm and t is time of harvest in days; and the subscripts 1 and 2 refer to the 14 and 21 days of harvesting after transfer, respectively.
At harvest, the entire root system dissected from the root-shoot junction was rinsed three times by DW and stored at –4°C until scan analysis. Each root was placed on a transparent plastic slide and thoroughly combed apart in DW. The desktop scanner (Epson Perfection V850 Pro) was used to scan the roots at a resolution of 400 dpi. The scanned images were analyzed using the WinRHIZO software (version 5.0) (Regent Instruments Inc., Quebec City, QC, Canada) to quantify root indexes as described (Freschet et al., 2020 (link)). The relative root growth rate (RRGR) was computed as a given equation (Samal et al., 2010 (link)).
Where, ln is natural log, TRL is total root length in cm and t is time of harvest in days; and the subscripts 1 and 2 refer to the 14 and 21 days of harvesting after transfer, respectively.
4
Root and Leaf Morphometric Analysis
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From each treatment group, 10 plants were randomly selected and separated into leaf and root fractions. The leaf area of the youngest fully developed leaf of each plant was measured. Root samples were placed in a rectangular glass dish with a thin layer of water (4–5 mm depth) to allow all roots to spread appropriately. Entire roots were scanned with an EPSON Transparency unit (Epson Perfection V700 Photo; Indonesia), and then analyzed with WinRHIZO software version 5.0 (Regent Instruments, Inc.; Canada) to calculate the total root length, total root surface area, total root volume, and average root diameter. Leaves and roots were washed with deionized water and dried at 70°C for 48 h to determine dry weight before being ground to determine the ion contents.
In addition, another six plants per replicate of each of the four treatments were harvested. Fresh roots of seedlings were separated and frozen immediately in liquid nitrogen before being stored at −80°C pending further analysis.
In addition, another six plants per replicate of each of the four treatments were harvested. Fresh roots of seedlings were separated and frozen immediately in liquid nitrogen before being stored at −80°C pending further analysis.
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