MCF10A, a human breast epithelial cell line (ATCC CRL-10317), was purchased from the Korean Cell Line Bank (Seoul, Korea). HEK293FT (referred to as 239FT), a human embryonic kidney cell line (ATCC PTA-5077), was kindly provided by Prof. H.G. Yoon (Yonsei University, Korea). MCF10A cells were grown in Dulbecco's Modified Eagle's Medium (DMEM, Welgene, Korea) supplemented with Nutrient Mixture F-12 (DMEM/F-12, Invitrogen, CA), 10% horse serum, 1% penicillin, 20 ng/mL of EGF, 0.5 mg/mL of hydrocortisone, 100 ng/mL of cholera toxin, and 10 μg/mL of insulin. 293FT cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS, Hyclone laboratories Inc. USA) and 5% penicillin. Both cell lines were grown in a humidified 5% CO2 incubator at 37°C. All media were changed every 2-3 days, and cells were subcultured at 1:5 to 1:10.
Mcf10a
Manufactured by Welgene
MCF10A is a non-tumorigenic human breast epithelial cell line derived from spontaneously immortalized cells. It is commonly used as a model for studying breast cancer and epithelial cell biology.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 10a, by American Type Culture Collection (2 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Dulbecco modified eagle medium (dmem), by Cytiva (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
Raw264, by American Type Culture Collection (1 mentions)
Cholera toxin, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Raw 264, by Welgene (1 mentions)
Rbl 2h3, by American Type Culture Collection (1 mentions)
Penicillin and streptomycin, by Welgene (1 mentions)
Horse serum, by Welgene (1 mentions)
Rbl 2h3, by Welgene (1 mentions)
Dulbecco s modified eagle s medium dmem, by Welgene (1 mentions)
2 protocols using mcf10a
1
Cultivation of MCF10A and HEK293FT Cell Lines
2
Culturing Diverse Cell Lines for Research
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The human breast epithelial cell line MCF 10A, murine macrophage cell line RAW 264.7, and rat basophilic leukemia mast cell line RBL-2H3 cells were purchased from American Type Culture Collection (ATCC, VA, USA). The MCF 10A cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, WELGENE, Korea) supplemented with 5% heat-inactivated horse serum (WELGENE, Korea), 10 µg/mL insulin (Sigma-Aldrich), 100 ng/mL cholera toxin (Sigma-Aldrich), 0.5 µg/mL hydrocortisone (Sigma-Aldrich), 20 ng/mL recombinant human epithermal growth factor (Sigma-Aldrich), and 1% penicillin and streptomycin (WELGENE) at 37 °C in a humidified atmosphere of 5% CO2. RAW 264.7 and RBL-2H3 cells were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS, WELGENE) and 1% penicillin and streptomycin at 37 °C in a humidified atmosphere of 5% CO2.
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