Anti β actin antibody 66009 1 ig

Safranal (≥90.0% purity) was purchased from Sigma Aldrich (St. Louis, MO, United States), dissolved in dimethyl sulfoxide (DMSO, Life Technologies, Carlsbad, CA, United States), and stored at -80°C. Propidium iodide (PI, P4170; Sigma-Aldrich), PrimeScript RT Reagent Kit (RR037A; TAKARA Biotechnology, Shiga, Japan), SYBR Green reagent (S-7563; Life Technologies), and SYBR Green Realtime PCR Master Mix (QPK-201; TOBOYO, Life Science, Osaka, Japan) were used. The antibodies for immunoblotting included those against CDK4 (#3830-1), CDK6 (#3524-1), and p21 (#3733-1) (Epitomics, Cambridge, United Kingdom). We acquired antibodies against p27 (sc528), IKKα/β (sc7607), α-tubulin (sc5286), and Skp2 (sc7164) from Santa Cruz Biotechnology (Dallas, CA, United States), while anti-β-actin antibody (#66009-1-Ig) was purchased from Proteintech (Wuhan, Hu Bei Province, China). The antibodies against p-Rb (Ser807/811, #9308), E2F1 (#3742), phospho-AKT (Ser473, #4060), AKT (#9272), phospho-IKKα/β (Ser176/180, #2697), phospho-IκBα (Ser32, #2859), IκBα (#4812), NF-κB p65 (#8242), and NF-κB2 (p100/52, #3017) were purchased from Cell Signaling Technology (Danvers, MA, United States), while those against lamin A/C (ab108595), CDK2 (ab32147), c-MYC (ab32072), and GAPDH (ab128915) were supplied by Abcam (Cambridge, United Kingdom).

An anti-Flag M2 affinity gel (A2220) and anti-Flag-HRP antibody (A8592) were purchased from Sigma (Missouri, USA). An anti-Myc antibody (AE010), an anti-V5 antibody (AE017) and an anti-β-tubulin antibody (AC030) were purchased from Abclonal (Wuhan, China). An anti β-Actin antibody (66009-1-Ig) was purchased from Proteintech (Wuhan, China). An anti-HA-HRP antibody (14031S) and a normal rabbit IgG antibody (2729s) were purchased from Cell Signaling Technology (Massachusetts, USA). A goat anti rabbit-HRP antibody (ZB-2301) and a goat anti mouse-HRP antibody (ZB-2305) were purchased from ZSGB-BIO (Beijing, China). Protein A/G PLUS-Agarose (sc-2003) was purchased from Santa Cruz Biotechnology (Texas, USA).

Apilimod (No. S6414) was bought from Selleck. Nigericin, MSU, and poly (A/T) were purchased from Sigma-Aldrich. Ultrapure LPS, MitoSOX, MitoTracker, DAPI, Lysotracker Green, MitoTracker Deep Red, MitoTracker Green were acquired from Invitrogen. MnTBAP was bought from Santa Cruz Biotechnology. The C3 toxin was a gift from Dr. Tengchuan Jin (University of Science and Technology of China, Hefei, China). CY-09 was synthesized from Dr. Xianming Deng (Xiamen University, Xiamen, China). MCC950 was bought from Selleck. Fluo-4, AM(HY-101896), ML-SI3 were purchased from MedChemExpress. BAPTA-AM(A1076) was bought from Sigma-Aldrich. Protein G agarose (16–266) was bought from Millipore. Anti-Flag (F2555) antibodies were from Sigma-Aldrich. The anti-mouse NLRP3 (AG-20B-0014) and the anti-mouse caspase-1 (AG-20B-0042) antibodies was from AdipoGen. The anti-mouse IL-1β antibody (AF-401-NA) was obtained from R&D Systems. The anti-β-actin antibody (66009-1-Ig) was from Proteintech Group. The anti-mouse ASC antibody (67824S) was from Cell Signaling Technology. The anti-mouse NEK7 antibody (ab133514) was from Abcam.