Mouse peripheral blood leukocytes were stained with antibodies for 30 minutes in the dark, including fluorescently-conjugated antibodies specific to CD45.2 (BD Biosciences; clone-30-F11), CD3 (Biolegend; clone-145–2C11), B220 (Biolegend; clone-RA3–6B2), CD25 (Biolegend; clone-PC61.5), CD8b (Biolegend; clone-YTS156.7.7), CD4 (Biolegend; clone-GK1.5), and NK1.1 (Biolegend; clone-PK136). Cells were then washed with FACS buffer (PBS, 2% FBS, 2mM EDTA) and resuspended in FACS buffer before performing multicolor flow cytometric analysis using a FACS CantoII machine (BD Biosciences). Flow cytometry data were analyzed using FlowJo software version 10 (Tree Star).
Clone yts156
Manufactured by BioLegend
Clone-YTS156.7.7 is a mouse monoclonal antibody that recognizes the CD28 antigen. CD28 is a co-stimulatory molecule expressed on T cells that is involved in T cell activation and survival. This antibody can be used for flow cytometry and other immunoassay applications.
Automatically generated - may contain errors
2 protocols using clone yts156
1
Multicolor Flow Cytometry of Mouse Leukocytes
2
Multicolor Flow Cytometry of Mouse Leukocytes
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