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Hp 8452a

Manufactured by Agilent Technologies
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The HP 8452A is a diode array spectrophotometer. It is designed to measure the absorbance of samples across a range of wavelengths in the ultraviolet and visible light spectrum.

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Lab products found in correlation

Fluoromax 4, by Horiba (2 mentions) Nano zs instrument, by Malvern Panalytical (1 mentions) Salicylic acid, by Merck Group (1 mentions) Gemini, by Agilent Technologies (1 mentions) 1100 series, by Agilent Technologies (1 mentions) Mpc k6, by Huber (1 mentions)

4 protocols using hp 8452a

1

Spectrophotometric Characterization of Compounds

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UV-VIS absorption spectra were recorded on a diode-array spectrophotometer (Agilent Hewlett-Packard, HP 8452A, Agilent Technologies Inc., Santa Clara, CA, USA) in 1 cm quartz cuvettes at room temperature. Emission measurements were acquired from dilute solutions (Abs max < 0.1) in 1 cm cuvettes at room temperature and with excitation at the sample absorption maximum. Corrected emission spectra were recorded with a research spectrofluorometer (Fluoromax-4, Horiba, Kioto, Japan).
Arias-Ramos N., Ibarra L.E., Serrano-Torres M., Yagüe B., Caverzán M.D., Chesta C.A., Palacios R.E, & López-Larrubia P. (2021). Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors. Pharmaceutics, 13(8), 1258.
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2

Characterization of Aptamer-Decorated Conjugated Polymer Nanoparticles

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Aptamer-decorated CPNs and nonconjugated CPNs were characterized by size and zeta potential by dynamic light scattering (DLS) using Malvern Zetasizer Nano ZS instrument (Herrenberg, Germany) equipped with a 633 nm laser. UV-VIS absorption spectra were recorded on a diode-array spectrophotometer (Agilent Hewlett-Packard, HP 8452A, Agilent Technologies Inc., Santa Clara, CA, USA) in 1 cm quartz cuvettes at RT. Emission measurements were acquired from dilute solutions (Abs max < 0.1) in 1 cm path length cuvettes at RT and with excitation at the sample absorption maximum. Corrected emission spectra were recorded with a research spectrofluorometer (Fluoromax-4, Horiba, Kyoto, Japan). Agarose gel electrophoresis was used to verify the successful conjugation of CPNs with aptamers.
Ibarra L.E., Camorani S., Agnello L., Pedone E., Pirone L., Chesta C.A., Palacios R.E., Fedele M, & Cerchia L. (2022). Selective Photo-Assisted Eradication of Triple-Negative Breast Cancer Cells through Aptamer Decoration of Doped Conjugated Polymer Nanoparticles. Pharmaceutics, 14(3), 626.
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3

Synthesis and Characterization of Bakuchiol Salicylate

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Bakuchiol salicylate was synthesized with salicylic acid (Sigma-Aldrich, St Louis, MO, USA) and bakuchiol (Sytheon, Boonton, NJ, USA and Abcam, Cambridge, MA, USA; two different suppliers were used to ensure reproducibility) by the modified Steglich esterification method using dichloromethane as solvent, with dicyclohexylcarbodiimide as condensing agent and 4-N,N-dimethylaminopyridine as catalyst. After solvent evaporation and removal of dicyclourea by filtration, the obtained residue was loaded onto a silicon dioxide (high-purity Fluka silica gel, pore size 60 Å, 70–230 mesh) column, and eluted with toluene/cyclohexane (1/1 v/v). The solvent was evaporated under vacuum, and the oily residue was analysed by infrared detection (Satellite FT-IR spectrophotometer; Mattson Instruments, Madison, WI, USA), nuclear magnetic resonance (200 MHz; Gemini; Varian, Palo Alto, CA, USA), diode array spectrophotometry (HP-8452A; Agilent Technologies, Palo Alto, CA, USA) and high-performance liquid chromatography (HPLC) (series 1100; Agilent Technologies) equipped with an injector (Rheodyne® 7725), binary pump, diode array–ultraviolet detection modules, C18 column (Xterra MS Waters; Milford, MA, USA) and ChemStation software. Samples dissolved in ethanol (10 μL) were eluted with 0.1% formic acid/acetonitrile gradient at 0.3 mL/min.
Ma S., Gobis K., Swindell W.R., Chaudhuri R., Bojanowski R, & Bojanowski K. (2017). Synthesis and activity of the salicylic acid ester of bakuchiol in psoriasis-surrogate keratinocytes and skin substitutes. Clinical and experimental dermatology, 42(3), 251-260.
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4

Spectroscopic Characterization of Photophysical Properties

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UV-vis absorption spectra were recorded using a HP 8452A spectrophotometer (Agilent) with Chemstation software. Fluorescence spectra were recorded by means of a custom-made spectrofluorometer using cw lasers (BeamQ, exc = 473 nm; Z-laser, exc = 532 nm) as excitation sources. Emitted photons were detected using an Andor ICCD camera coupled to a spectrograph.
All the emission spectra registered were corrected by the wavelength dependence of the spectral response of the detection system. In all the cases spectroscopic quality solvents and 1-cm quartz cuvettes were used. Temperature was controlled using a refrigerated circulator bath (Huber MPC-K6) connected to the sample holder. Fluorescence quantum yields were determined using the standard method 55 for highly diluted solutions of the compounds of interest to prevent selfabsorption processes (absorption < 0.05 at the excitation wavelength) and relative to N,N'-bis(1hexylheptyl)perylene-3,4,9,10 tetracarboxybismide in acetonitrile (Φfl=1 56 ) or N,N'-bis(butyl)-1,6,7,12-tetra-(4-tert-butylphenoxy)perylene-3,4:9,10-tetracarboxylic diimide in CH2Cl2 (Φfl =1 57 ). The same excitation wavelength was used for both the sample of interest and the standard in all these measurements.
Villabona M., Benet M., Mena S., Al-Kaysi R.O., Hernando J, & Guirado G. (2018). Multistimuli-Responsive Fluorescent Switches Based on Spirocyclic Meisenheimer Compounds: Smart Molecules for the Design of Optical Probes and Electrochromic Materials. The Journal of organic chemistry, 83(16).
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