Axiocam 305 color high speed camera

Pharmacological effects of the peptides and saline solution on DAD were assessed on days 7, 14 and 30.
After the animals were euthanized, the lungs were extracted and filled with 10% neutral formalin solution. The tissue specimens were rinsed in running water, dehydrated in an ascending alcohol series and embedded in paraffin. Then, 4–5 µm paraffin sections were stained with hematoxylin and eosin and examined by ordinary light microscopy using an AxioScope A1 (Carl Zeiss, Oberkochen, Germany). Microphotographs of the histological sections were made with Axiocam 305 color high-speed camera (Carl Zeiss, Oberkochen, Germany) and the software ZEN 2.6 lite (Carl Zeiss, Oberkochen, Germany). Histological examination included the assessment of the following morphological characteristics: peribronchial and perivascular infiltration with the mononuclear cells, infiltration of alveolar walls and ducts with the mononuclear cells, sites of pulmonary collapse and the presence/absence of necrotic foci.
The extent of different inflammatory manifestations in the lungs was evaluated using a semiquantitative scoring scale: 0—none (within the normal range); 1—minimal; 2—mild; 3—moderate; 4—severe, tissue alterations are noticeable, but there is a potential for increase in severity; 5—very severe, the maximal extent of alterations, characterizing the total lobe injury.

Histological studies of rat spinal cord were performed at days 1, 7, 14, 21, 30 and 60, as described previously [12 (link),13 (link)]. Briefly, samples of the rat spinal cord encased in bone corresponding to the length of three vertebrae (Th13 was the vertebra of the surgical approach, and Th12 and L1 were the two adjacent vertebrae) were fixed in 10% neutral buffered formalin, rinsed in tap water and processed for decalcification in Trilon B at room temperature for 12–16 days. The biomaterial was oriented for further microtomy in the sagittal planes. Hematoxylin and eosin staining was used for serial 4–5 μm thick paraffin-embedded sections. The sections were examined by the standard light microscopy with an Axio Scope.A1 microscope (Carl Zeiss, Oberkochen, Germany). Photomicrographs of the histological sections were made with an Axiocam 305 color high-speed camera (Carl Zeiss, Oberkochen, Germany).