For DNA extraction, strains were thawed and grown in blood agar at 28 °C for 48 h. Colonies were collected and submitted to the Maxwell® 16 MDx Research Instrument (Promega, USA) following the manufacturer’s instructions. Genomic DNA were quantified using a Qubit 2.0 Fluorometer (Life Technologies, Thermo Scientific, USA). The strain SA2037 (link) was re-sequenced by our group27 (link) on a MiSeq sequencer (Illumina, USA) using a Nextera™ DNA Library Prep Kit. All other strains were sequenced on an Ion Torrent Personal Genome Machine™ (PGM) (Life Technologies) with the Ion PGM Sequencing 200 bp Kit following the manufacturer’s recommendations.
Maxwell 16 mdx research instrument
Manufactured by Promega
Sourced in United States
The Maxwell® 16 MDx Research Instrument is a compact, automated nucleic acid extraction system designed for efficient and consistent sample processing. It utilizes magnetic bead-based technology to extract DNA, RNA, or miRNA from a variety of sample types. The instrument is capable of processing up to 16 samples simultaneously, providing a streamlined and standardized approach to nucleic acid purification.
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2 protocols using maxwell 16 mdx research instrument
1
Bacterial Genome Extraction and Sequencing
2
Circadian Rhythms in Drosophila Gene Expression
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Gene expression was measured in head samples of flies collected at light-on (ZT0) and light-off (ZT12) times. Flies (3–4 days old) were trained for 3 days in a 12:12 LD cycle at 25 °C, after which male heads were collected in liquid nitrogen at ZT0 or ZT12. RNA was extracted using a Maxwell 16 MDx Research Instrument (Promega), combined with the Maxwell 16 Tissue Total RNA purification kit (AS1220, Promega). RNAseq library preparation and sequencing was carried by Glasgow Polyomics using an IlluminaNextseq500 platform.
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