Ha tag rabbit polyclonal antibody

TOPK and β-actin were purchased from Santa Cruz Technology, Inc (Santa Cruz, CA, USA). Phospho-Src (Tyr416) (D49G4) Rabbit mAb, Src (32G6) Rabbit mAb, Histone H3 (D1H2) XP Rabbit mAb, phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb, and HA-Tag Rabbit polyclonal antibody were purchased from Cell Signaling Technology, Inc (Boston, MA, USA). Ubiquitin Rabbit polyclonal antibody was purchased from Proteintech Group, Inc (Chicago, USA). His-Tag Mouse monoclonal antibody (4E6) and HA-Tag Mouse monoclonal antibody (1B10) were obtained from Pregene, Inc (Beijing, China). HRP-labeled Goat anti Mouse IgG (H+L) and Goat anti Rabbit IgG (H+L) were purchased from EarthOx, LLC (San Francisco, CA, USA). Donkey anti-Rabbit IgG (H+L) Alexa Fluor^® 546 Red and Donkey anti-Mouse IgG (H+L) Alexa Fluor^® 488 Green were purchased from Invitrogen, Inc (Carlsbad, CA, USA). Phospho-TOPK at Y74 or Y272 antibodies were prepared by Abgent, Inc (Suzhou, China). All antibodies were used following the instructions of the respective manufacturers. The small hairpin RNA constructs against Src (5′-GACAGACCTGTCCTTCAAGAA-3′) used in this study was from the BioMedical Genomics Center at the University of Minnesota. Dasatinib was purchased from Selleckchem, Inc (Houston, TX, USA).

The TOPK mouse mAb antibody (sc-293028) was purchased from Santa Cruz Technology, Inc. (Santa Cruz, CA). TOPK rabbit mAb (#4942), phospho-c-Raf (Ser338) (56A6) rabbit mAb(#9427), Raf mouse mAb (#12552), ERK1/2 rabbit mAb (#4695), p-ERK1/2(Thr202/Tyr204) rabbit mAb (#4649), histone H3 (D1H2) XP rabbit mAb (#4499), phospho-histone H3 (Ser10) (D2C8) XP rabbit mAb (#53348), histone H3 rabbit mAb (#4499), MET Rabbit mAb (#8198), p-MET Rabbit mAb (#3077), and HA tag rabbit polyclonal antibody (#3724) were purchased from Cell Signaling Technology (CST), Inc.((Danvers, MA). The β-actin mouse monoclonal antibody (#3700) and secondary antibody against mouse and rabbit were purchased from CST. Anti-HGF antibody (ab178395) was purchased from Abcam, Inc. (Cambridge, MA). The phospho-TOPK at S32 and Y74 antibodies was prepared by Abgent, Inc. (Suzhou, JS). All antibodies were used following the manufacturers’ instructions. Vector plasmids were transfected using Lipofectamine 2000 from Invitrogen, Inc. (Carlsbad, CA). The shRNA constructs against ERK2 were designed by the BioMedical Genomics Center at the University of Minnesota. Sorafenib, OTS964 and SCH772984 were purchased from Selleckchem, Inc. (Houston, TX). G418 and puromycin were purchased from Sigma-Aldrich (St. Louis, MO) and Solarbio, Inc. (Beijing, BJ). EGF and HGF was purchased from R&D Systems, Inc. (Minneapolis, MN).