Cell total protein extraction kits

Manufactured by Beyotime

Sourced in China

The Cell Total Protein Extraction Kits are designed to efficiently extract total cellular proteins from various cell types. The kits include reagents and protocols to lyse cells and solubilize the proteins, allowing for the collection of a comprehensive protein sample for further analysis.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Beyotime (4 mentions) Membrane protein extraction kit, by Beyotime (3 mentions) Celltiter 96tm aqueous one solution cell proliferation assay, by Thermo Fisher Scientific (2 mentions) 1 19 dioctadecyl 3 3 39 39 tetramethylindodicarbocyanine perchlorate did, by Biotium (2 mentions) Goat anti rabbit igg h l alexa fluor 488 ab150077, by Abcam (2 mentions) Tnf α and il 6 elisa kit, by Solarbio (2 mentions) Hrp conjugated anti rabbit igg, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Bicinchoninic acid assay bca protein assay kit, by Beyotime (2 mentions) 4 dimethylaminopyridine dmap, by Maclin Biochemical Technology (1 mentions) N n carbonyldiimidazole cdi, by J&K Scientific (1 mentions) Naclo, by Maclin Biochemical Technology (1 mentions) 1 19 dioctadecyl 3 3 39 39 tetramethylindodicarbocyanine perchlorate, by Biotium (1 mentions) Hydrogen peroxide h2o2, by Chron Chemicals (1 mentions) Lipopolysaccharide lps, by Solarbio (1 mentions) Lysotracker green, by Yeasen (1 mentions) N acetyl l cysteine, by Beyotime (1 mentions) Rpmi 1640 medium, by Sartorius (1 mentions) Membrane and cytosol protein extraction kit, by Beyotime (1 mentions) Dulbecco s modified eagle s medium dmem high glucose, by Sartorius (1 mentions)

7 protocols using cell total protein extraction kits

Synthesis and Characterization of Boronic Acid-Dextran Conjugate

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4-(Hydroxymethyl) phenylboronic acid pinacol ester (PBAP), dextran with molecular weight (MW) of 5000 g/mol, and ferrous perchlorate were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). 4-Dimethylaminopyridine (DMAP) and NaClO were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). N,N’-Carbonyldiimidazole (CDI) was purchased from J&K Chemical Co., Ltd. (Shanghai, China). RAP was purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Deuterated dimethyl sulfoxide (DMSO-d₆) and deuterated methanol (methanol-d₄) were purchased from Sun Chemical Technology Co., Ltd (Shanghai, China). Hydrogen peroxide (H₂O₂) was purchased from ChengDu Chron Chemicals Co., Ltd (Chengdu, China). DAPI and cell total protein extraction kits were purchased from Beyotime Institute of Biotechnology Co., Ltd (Jiangsu, China). 1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, USA). The CellTiter 96^TM AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Thermo Fisher Scientific (SanJose, CA, USA).

Tang D., Wang Y., Wijaya A., Liu B., Maruf A., Wang J., Xu J., Liao X., Wu W, & Wang G. (2021). ROS-responsive biomimetic nanoparticles for potential application in targeted anti-atherosclerosis. Regenerative Biomaterials, 8(4), rbab033.

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Nanoparticle Fabrication and Characterization

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RAP and PLGA (MW 90000, 50:50) were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, Chia). 1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, US). DiO, DAPI, Cell Total Protein Extraction kits and Membrane Protein Extraction kits were supplied by Beyotime Institute of Biotechnology (Jiangsu, China). The CellTiter 96^TM AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Thermo Fisher Scientific (SanJose, CA, USA). Lipopolysaccharide (LPS) was purchased from Solarbio (Beijing, China). The mouse glycoprotein ELISA kit, mouse TNF-α ELISA kit and mouse IL-6 ELISA kit were purchased from Wuhan Colorful Gene Biological Technology Co., Ltd. (Wuhan, China). LysoTracker Green was purchased from Yeasen Biotech Co. Ltd. (Shanghai China). Ultrapure water with a resistivity of 18.2 MΩ·cm was used throughout the experiments.

Wang Y., Zhang K., Li T., Maruf A., Qin X., Luo L., Zhong Y., Qiu J., McGinty S., Pontrelli G., Liao X., Wu W, & Wang G. (2021). Macrophage membrane functionalized biomimetic nanoparticles for targeted anti-atherosclerosis applications. Theranostics, 11(1), 164-180.

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DiD-Based Fluorescence Imaging Assay

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1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, USA). DAPI, cell total protein extraction kits, and N-acetyl-l-cysteine (NAC) were provided by Beyotime Institute of Biotechnology (Jiangsu, China). Antibodies against CD31 (ab28364) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) were obtained from Abcam. l-Ascorbic acid (VC) was purchased from Aladdin (Shanghai, China). Malondialdehyde (MDA), superoxide dismutase (SOD), and monocyte chemotactic protein 1 (MCP-1) enzyme-linked immunosorbent assay (ELISA) kits were provided by KeyGen Biotech (Nanjing, China).

Qin X., Zhang K., Qiu J., Wang N., Qu K., Cui Y., Huang J., Luo L., Zhong Y., Tian T., Wu W., Wang Y, & Wang G. (2021). Uptake of oxidative stress-mediated extracellular vesicles by vascular endothelial cells under low magnitude shear stress. Bioactive Materials, 9, 397-410.

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Characterization of LA/TCS@PLGA-NP Protein by SDS-PAGE

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The LA/TCS@PLGA-NP protein was characterized by SDS–PAGE. The envelope proteins of L. acidophilus, L. acidophilus envelope fragments and LA/TCS@PLGA-NPs were extracted by cell total protein extraction kits (Beyotime). The extracted proteins were run on a 12% SDS–PAGE gel in running buffer using a BIO-RAD electrophoresis system at 70 V for 0.5 h and then at 140 V for 1 h. Finally, the SDS–PAGE gel was stained with SimplyBlue for visualization.
The LA/TCS@PLGA-NP protein was further detected by quantitative analysis according to Feng et al. [58 (link)]. The LA/TCS@PLGA-NPs were lyophilized and weighed. The protein content of LA/TCS@PLGA-NPs was measured by a BCA protein assay kit. The protein loading yield, which is defined as the weight ratio of immobilized proteins on the nanoparticles, was calculated.

Weng L., Wu L., Guo R., Ye J., Liang W., Wu W., Chen L, & Yang D. (2022). Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries. Journal of Nanobiotechnology, 20, 356.

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Peptide-based Nanomaterial Synthesis and Characterization

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Peptides (LFVRRGQPFTIILYFC) was purchased from Nanjing Taiye Co. Ltd. (Nanjing, China). HO‐PEG_2K‐Mal was purchased from Pengshuo Co. Ltd. (Shanghai, China). OC and LVT were purchased from Macklin Co. Ltd. (Shanghai, China). DiD was purchased from Biotium Inc (Fremont, USA). FITC, Cy5, and D, l‐dithiothreitol (DTT) were provided by Sigma–Aldrich (MO, USA). NAP‐5 columns were obtained from GE Healthcare (Pittsburgh, PA). 4',6‐diamidino‐2‐phenylindole (DAPI) and cell total protein extraction kits were provided by Beyotime Institute of Biotechnology (Jiangsu, China). Antibodies against CD31 (ab28364), α‐SMA (ab5694), and goat anti‐rabbit IgG H&L (Alexa Fluor 488) (ab150077) were obtained from Abcam. Fetal bovine serum (FBS), RPMI 1640 medium, and Dulbecco's modified Eagle's medium (DMEM) high glucose were purchased from Biological Industries (Chongqing, China). Membrane and cytosol protein extraction kit was purchased from Beyotime (Shanghai, China).

Qin X., Zhu L., Zhong Y., Wang Y., Wu G., Qiu J., Wang G., Qu K., Zhang K, & Wu W. (2023). Spontaneously Right‐Side‐Out‐Orientated Coupling‐Driven ROS‐Sensitive Nanoparticles on Cell Membrane Inner Leaflet for Efficient Renovation in Vascular Endothelial Injury. Advanced Science, 10(6), 2205093.

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Platycodon Polysaccharide Encapsulation

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4-hydroxymethyl phenylboronic acid pinacol ester (4-PBAP) and N, N'-carbonyl diimidazole (CDI) were purchased from Aladdin (China). Platycodon grandi orum polysaccharides were supplied by Shanghai Winherb Medical Technology Co., Ltd. 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyaine iodide (DiR) was obtained from Beijing Bioss Biotechnology Co. DAPI, Cell Total Protein Extraction kits, Bicinchoninic Acid Assay (BCA) protein assay kit and Membrane Protein Extraction kits were supplied by Beyotime Institute of Biotechnology (China). TNF-α and IL-6 ELISA kit were purchased from Beijing Solarbio Technology Co., Ltd. (China). Antibody CCR2 (anti-rabbit, #ABP53395) special for mouse were purchased from Abbkine (China), with a dilution ratio of 1:1000 for antibody. Antibodies β-Actin (#4967S) and HRPconjugated anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (USA), with a dilution ratio of 1:1000 for both antibodies during experimental use. All other chemical reagents were supplied by Aladdin (China). All biological reagents, and other cell culture medium were used as received following our previous protocols.

Li Y., Guo C., Chen Q., Su Y., Guo H., & Chen D. (2021). Lung-targeted Macrophage Biomimetic Nanocarriers Based on Platycodon Grandiflorum Polysaccharides for Calming Cytokine Storm in Pneumonia.

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Platycodon Polysaccharide Encapsulation

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Li Y., Guo C., Chen Q., Su Y., Guo H., & Chen D. (2021). Bionanosystem Based on Platycodon Grandifloras “Guides the Medicine Upward” for Targeting Lung Inflammation to Calm the Cytokine Storm.

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