RNA extraction, RNA retrotranscription, real-time PCR and western blot analysis were performed as previously described (Harlan, Pehar, Killoy, & Vargas, 2019 (link)). Membranes were incubated overnight with one of the following antibodies: FABP7 (Thermo Fisher, Cat#: PA5-24949, TL2690549A), Actin (Sigma, A5441, lot: 061M4808) or GADPH (Sigma, SAB2100894, lot: QC9353). Image acquisition was performed in a chemiluminescent western blot scanner (Li-Cor) or exposed on Kodak BioMax Light film. Quantifications were performed using the Image Studio Software (Li-Cor) or the ImageJ Software (NIH). ChIP was performed with the SimpleChIP enzymatic chromatin IP kit (Cell Signaling) using an NF-κB p65 antibody (Cell Signaling, 8242, lot: 13) and analyzed by real-time PCR.
Fabp7
Manufactured by Thermo Fisher Scientific
FABP7 is a recombinant protein that is part of the Fatty Acid Binding Protein family. It is used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Gadph, by Merck Group (1 mentions)
Image studio software, by LI COR (1 mentions)
Nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
Biomax light film, by Kodak (1 mentions)
Simplechip enzymatic chromatin ip kit, by Cell Signaling Technology (1 mentions)
Chemiluminescent western blot scanner, by LI COR (1 mentions)
Twist1, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene fluoride (pvdf), by Beyotime (1 mentions)
Gapdh, by Abcam (1 mentions)
Ecl reagent, by Thermo Fisher Scientific (1 mentions)
E cadherin, by Abcam (1 mentions)
Snail, by Abcam (1 mentions)
Goat anti rabbit igg h l hrp, by Abcam (1 mentions)
Rabbit anti mouse igg h l hrp, by Abcam (1 mentions)
Ripa solution, by Beyotime (1 mentions)
2 protocols using fabp7
1
Protein Extraction and Analysis Workflow
2
Western Blot Analysis of Epithelial-Mesenchymal Transition Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BC tissues and cells were lysed in radioimmunoprecipitation assay
buffer (RIPA) solution (Beyotime) contained protease inhibitors, and the proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to the polyvinylidene fluoride (PVDF, Beyotime) membranes. After being sealed in 5% skim milk for 2 hours, the membranes were hatched together with the primary antibodies targeting Snail (1:1000, Abcam), Twist1 (1:1000, Thermo Fisher Scientific), E‐cadherin (1:500, Abcam), FABP7 (1:500, Thermo Fisher Scientific), or GAPDH (1:2000, Abcam) at 4°C overnight. The samples were then incubated with secondary antibodies goat anti‐rabbit IgG H&L (HRP) or rabbit anti‐mouse IgG H&L (HRP) (1:4000, Abcam) for 1 hour. The protein bands were examined by using an ECL reagent (Thermo Fisher Scientific).
buffer (RIPA) solution (Beyotime) contained protease inhibitors, and the proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to the polyvinylidene fluoride (PVDF, Beyotime) membranes. After being sealed in 5% skim milk for 2 hours, the membranes were hatched together with the primary antibodies targeting Snail (1:1000, Abcam), Twist1 (1:1000, Thermo Fisher Scientific), E‐cadherin (1:500, Abcam), FABP7 (1:500, Thermo Fisher Scientific), or GAPDH (1:2000, Abcam) at 4°C overnight. The samples were then incubated with secondary antibodies goat anti‐rabbit IgG H&L (HRP) or rabbit anti‐mouse IgG H&L (HRP) (1:4000, Abcam) for 1 hour. The protein bands were examined by using an ECL reagent (Thermo Fisher Scientific).
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