Behavioral changes were monitored for 1 h in week 9 after a single administration of Minocycline hydrochloride (30 mg/kg). Minocycline is a microglial activation inhibitor (Sigma–Aldrich Corp. St. Louis, MO, USA). In week 10, the selective p38 MAPK inhibitor, SB 203580 hydrochloride (50 lg/kg) (Tocris Bioscience, R&D Systems, Inc., Minneapolis, MN, USA), was given prior to behavioral testing. Both drugs were dissolved in normal saline and given by intraperitoneal (i.p.) injection. Normal saline served as the vehicle control.
Sb 203580 hydrochloride
Manufactured by Bio-Techne
Sourced in United States
SB 203580 hydrochloride is a kinase inhibitor that selectively inhibits the p38 mitogen-activated protein kinase (MAPK) pathway. It is commonly used as a research tool to study the role of the p38 MAPK pathway in cellular processes.
Automatically generated - may contain errors
3 protocols using sb 203580 hydrochloride
1
Minocycline and SB 203580 Behavioral Effects
2
Cellular Responses to Replication Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All treatments, including APH, HU, UV, ATRi, ATMi, p38i and CHK1i, were added into fresh ESC medium and the cells were kept at 37°C under 3% O2 tension overnight (16–17 hr) unless otherwise indicated. APH was used at concentrations ranging from 0.4 to 6 µM as indicated. Similarly, HU was used at 0.1 mM - 0.2 mM. ATMi (KU-55933) and ATRi (VE 822) were used at the concentrations of 10 µM and 1 µM respectively. The CHK1 inhibitors LY2603618 and UCN-01 were used at the concentrations of 200 nM and 100 nM, respectively. UV radiation exposure was performed at the dosage of 5 J/m2 or 10 J/m2. For induction of ETAA1-AAD expression, Dox was used at the concentration of 1 µg/mL for 48 hr. SB 203580 hydrochloride (Tocris Bioscience) and SB 239063 (Tocris Bioscience) were used at the concentration of 5 and 10 µM.
3
Inhibitors for Multimodal Experiments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following inhibitors were used for in vitro, in vivo, and ex vivo experiments: AZD4547 (Selleck Chemicals), PD173074 (Selleck Chemicals), BLU9931 (Selleck Chemicals), SB203580 hydrochloride (Tocris Bioscience, Bio-Techne) (a selective inhibitor for p38 MAPK) (59) , and dasatinib and quercetin (Selleck Chemicals).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!