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Sureselect human all exon v4 or v5

Manufactured by Agilent Technologies
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The SureSelect Human All Exon v4 or v5 is a DNA library preparation kit designed for targeted enrichment of human exonic regions. It enables the selective capture and sequencing of the protein-coding regions of the human genome.

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Lab products found in correlation

Hiseq 2500, by Illumina (1 mentions) Hiseq 2000, by Illumina (1 mentions)

Spelling variants of this product

SureSelect Human All Exon V5 (58 citations) SureSelect Human All Exon V4 (35 citations) SureSelect Human All Exon V4 or V5 kit (5 citations)

2 protocols using sureselect human all exon v4 or v5

1

Exome Sequencing Protocol for Genetic Analysis

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Genomic DNA was captured using SureSelect Human All Exon v4 or v5 (Agilent Technologies, Santa Clara, CA), and sequenced on an Illumina HiSeq2500 (Illumina, San Diego, CA) with 101 bp paired‐end reads. Exome data processing, variant calling, and variant annotation were performed at Department of Human Genetics, Yokohama City University Graduate School of Medicine as described previously.21
Akita T., Aoto K., Kato M., Shiina M., Mutoh H., Nakashima M., Kuki I., Okazaki S., Magara S., Shiihara T., Yokochi K., Aiba K., Tohyama J., Ohba C., Miyatake S., Miyake N., Ogata K., Fukuda A., Matsumoto N, & Saitsu H. (2018). De novo variants in CAMK2A and CAMK2B cause neurodevelopmental disorders. Annals of Clinical and Translational Neurology, 5(3), 280-296.
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2

Exome Sequencing for KCNB1 Mutation Detection

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Genomic DNA was captured using SureSelect Human All Exon v4 or v5 (Agilent Technologies), and sequenced on an Illumina HiSeq2000 (Illumina) with 101 bp paired-end reads. Exome data processing, variant calling, and variant annotation were performed as described previously36 (link). For detecting KCNB1 mutations in WES data, we focused on rare nonsynonymous KCNB1 variants absent in dbSNP137 (http://www.ncbi.nlm.nih.gov/projects/SNP/), Exome Variant Server database (http://evs.gs.washington.edu/EVS/), and in our in-house 575 control exomes. These KCNB1 variants were annotated using GenBank accession number NM_004975.2. Segregation of all candidate KCNB1 mutations was examined by Sanger sequencing using trio samples (patients and their parents). In families showing de novo mutations, parentage was confirmed by analyzing 12 microsatellite markers.
Saitsu H., Akita T., Tohyama J., Goldberg-Stern H., Kobayashi Y., Cohen R., Kato M., Ohba C., Miyatake S., Tsurusaki Y., Nakashima M., Miyake N., Fukuda A, & Matsumoto N. (2015). De novo KCNB1 mutations in infantile epilepsy inhibit repetitive neuronal firing. Scientific Reports, 5, 15199.
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