The proanthocyanidin standards including the galloylated analogs were purchased from ChromaDex Inc. (Irvine, CA, USA), except for proanthocyanidin B1, which was purchased from Quality Phytochemicals LLC (East Brunswick, NJ, USA). The HPLC grade, CH3OH, CH3CN, H2O, and EtOH were from Honeywell (Morris Plains, NJ, USA).
Ch3cn
Manufactured by Honeywell
Sourced in United States
CH3CN is a chemical compound that is commonly used as a solvent in various laboratory applications. It is a colorless, volatile liquid with a characteristic acetonitrile odor. This product is often utilized in analytical and organic chemistry techniques, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC).
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade, by Honeywell (1 mentions)
Ch3oh, by Honeywell (1 mentions)
Dimethylformamide, by Thermo Fisher Scientific (1 mentions)
Ammonium peroxydisulfate, by Merck Group (1 mentions)
Pyrrole, by Merck Group (1 mentions)
H3po4, by Carlo Erba (1 mentions)
4 dodecylbenzenesulfonic acid, by Merck Group (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
Naphthalene, by Merck Group (1 mentions)
Methyl orange, by Merck Group (1 mentions)
E line spectrometer, by Agilent Technologies (1 mentions)
Ls 45 fluorescence spectrometer, by PerkinElmer (1 mentions)
Sunrise absorbance reader, by Tecan (1 mentions)
Avance 300 mhz, by Bruker (1 mentions)
Model ea 1108, by Carlo Erba (1 mentions)
3 protocols using ch3cn
1
Proanthocyanidin Standards Procurement
2
Synthesis of Pyrrole-Functionalized PAN Nanocomposites
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PAN (MW 50 000–70 000 Da, 99%) was purchased from Sarchem Laboratories Inc., New Jersey. Pyrrole (98%), ammonium peroxydisulfate (APS), 4-dodecylbenzene sulfonic acid (DBSA) 99%, HCl 12 M, Rhodamine B (RB), Methyl Orange (MO), and naphthalene were purchased from Sigma Aldrich, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) from Enzo Life Sciences Inc., New York. Dimethylformamide (DMF) was supplied by Fisher Scientific, H3PO4 (85%) by Carlo Erba, CH3CN (Chromasolv for HPLC, purity ≥ 99.9%) by Honeywell. All the chemicals were used without further purification. Water was purified with a Milli-Q plus apparatus (TOC = 2 ppb, conductivity 18.2 MΩ cm). The commercial multiwalled carbon nanotubes employed were Nanocyl™ NC7000 industrial grade, purchased from Nanocyl S.A., Belgium. Their reported features were: 18 outer mean diameter 9.5 nm, average length 1.5 μm, specific surface area 475 m2 g−1, amorphous carbon pyrolytically deposited on the outer layer, C purity 90%, and metal oxides content 10% (1% transition metals). Reported impurities, determined by inductively coupled plasma mass spectrometry (ICP-MS), were: Al (5.9 wt%), Fe (0.5 wt%), and Co (0.2 wt%). This material was used without any further purification and is referred to in this article as CNT(NP), where NP stands for NanoParticles or Non-Purified.
3
Characterization of Novel Compounds
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CQDP, calf thymus DNA (CT-DNA), buffers and solvents were used as received from Sigma-Aldrich Co. All other commercial reagents were also used without further purification. The NMR spectra of the compounds were recorded in CH2Cl2-d2 and DMSO-d6 solution in Bruker AVANCE 300 MHz or 500 MHz spectrometers. IR spectra were recorded with a Perkin Elmer Series 100 spectrometer in the 4000–400 cm−1 region, ultraviolet-visible (UV-Vis) spectra on a HP 8453 diode array spectrometer and fluorescence spectra on a Perkin Elmer LS45 fluorescence spectrometer. C, H and N analyses were performed using a Carlo Erba Model EA1108 elemental analyzer. ESI (+) MS spectra were obtained by direct infusion in a QTOF Bruker Impact II Mass Spectrometer in positive ion mode, utilizing CH3CN (LC/MS grade from Honeywell; B&J Brand) as the solvent. Electron Paramagnetic Resonance (EPR) spectra were performed on a Varian E-Line spectrometer. In the β-hematin formation inhibition assay plates were centrifuged using a Thermo Scientific IEC CL 30 centrifuge and measured in a Tecan Sunrise Absorbance Reader at 405 nm.
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