1-Methyl-4-phenylpyridium (MPP⁺) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dichlorofluorescein diacetate (DCF-DA), DAPI (4′,6-diamidino-2-phenylindole), Alexa Floure 488, and Alexa Flour 568 were obtained from Invitrogen (Portland, OR, USA). Western blot detection reagent (ECL solution) was supplied by Advansta Inc. (San Jose, CA, USA). MHY2699 was synthesized by Prof. Hyung Ryong Moon (College of Pharmacy, Pusan National University, Busan, Korea), as described elsewhere [18 (link)].
1 methyl 4 phenylpyridium mpp
Manufactured by Merck Group
Sourced in United States
1-Methyl-4-phenylpyridium (MPP+) is a chemical compound used in laboratory research. It serves as a core functional tool for scientific investigations. The detailed description of its intended use and applications is not available in this response, as per the instructions provided.
Automatically generated - may contain errors
2 protocols using 1 methyl 4 phenylpyridium mpp
1
Novel Compound Synthesis and Assays
2
Exendin-4 and Linagliptin Enhance Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell viability was assessed by cell counting kit-8 (CCK-8; Shangbao, Shanghai, China). SH-SY5Y and BV2 cells were seeded into a 96-well plate and cultured for 24 hours. Then, the cells were treated with 1, 10, 100 nM, and 1 µM exendin-4 and linagliptin for 30 minutes, followed by treatment with 3 mM 1-methyl-4-phenylpyridium (MPP (+); (Sigma-Aldrich). After culturing for 24 hours, 10 µL enhanced CCK-8 reagent diluted in 100 µL serum-free medium was added to each well. Then, the 96-well plate was incubated at 37°C for 2 hours. The optical density (OD) value was measured at 490 nm using a microplate reader. Cell viability (%) = (OD of treated cells – blank OD)/(OD of control cells – blank OD) × 100.
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