All C. albicans strains used in this study were generated previously (Moyes et al., 2016 (link)) and are listed in Table 1 . C. albicans cells were cultured in liquid yeast peptone dextrose (YPD), consisting of 1% (w/v) yeast extract (Oxoid), 2% (w/v) Peptone (Melford), 2% (w/v) dextrose (BDH Chemicals) and 1.5% (w/v) agar (Melford) at 30°C overnight in a shaking incubator (180 rpm) to reach saturation. Cultures were washed in sterile PBS and adjusted to the required cell density. For cell damage, ELISA and western blotting assays, epithelial cells were infected with C. albicans strains at a multiplicity of infection (MOI) of 5 for 6 h and a MOI of 0.01 for 24 h. Following infection, cells were cultured at 37°C, 5% CO2.
Yeast peptone dextrose
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Yeast peptone dextrose is a type of culture medium used for the growth and cultivation of microorganisms, particularly yeasts. It provides the necessary nutrients, including carbon, nitrogen, and other essential elements, to support the growth and proliferation of yeast cells.
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Lab products found in correlation
2 protocols using yeast peptone dextrose
1
Candida albicans Cytotoxicity Assay
2
Cultivation and Biofilm Formation of Candida auris
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C. auris strain (BJCA001) used in this study was gifted from Professor Guanghua Huang [41 (link)]. This strain was routinely grown overnight at 30°C in yeast peptone dextrose (YPD) medium, which consists of 1% yeast extract (Oxoid, Basingstoke, England), 2% peptone (Solarbio, Beijing, China) and 2% dextrose (Solarbio). For the biofilm of C. auris construction, C. auris was activated on 9-mm diameter Petri dishes using Sabouraud dextrose agar (SDA), containing 25% dextrose, 1% peptone (Solarbio) and 1.5% agar (Solarbio). The formation of biofilms of C. auris was on RPMI 1640 medium, which consisted of 1.04% RPMI 1640 powder (Gibco, USA), 3.45% MOPS and 2% dextrose.
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