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2 protocols using petunidin 3 o glucoside chloride

1

Comprehensive Phytochemical Analytical Protocol

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Cyanidin-3-O-glucoside chloride, peonidin-3-O-glucoside chloride, delphinidin-3-O-glucoside chloride, petunidin-3-O-glucoside chloride, malvidin-3-O-glucoside chloride, protocatechuic acid, chlorogenic acid, morin, rutin, resveratrol, caffeic acid, p-coumaric acid, (+)-catechin, gallic acid, (−)-epicatechin, quercetin, ferulic acid, kaempferol, syringic acid, salicylic acid, vanillic acid, and 4-methyl-2-pentanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol (HPLC grade), acetonitrile (HPLC grade), and formic acid (HPLC grade) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Deionized water was obtained using a Milli-Q System (Millipore, Billerica, USA). 0.22 μm and 0.45 μm pore size syringe filters were purchased from Jinteng (Tianjin, China).
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2

Quantitative Analysis of Blueberry Polyphenols

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Commercial standards of cyanidin-3-O-glucoside chloride, malvidin-3-O-glucoside chloride, peonidin-3-O-glucoside chloride, petunidin-3-O-glucoside chloride, gallic acid, caffeic acid, ferulic acid, ethyl gallate, taxifolin, chlorogenic acid, 4-hydroxybenzoic acid, 3-hydroxybenzaldehyde, hippuric acid, 3-hydroxyhippuric acid, isovanillin, 4-hydroxyphenylacetic acid, 3-hydroxyphenylpropionic acid, 3-methoxyphenylacetic acid, vanillic acid, homovanillic acid, syringic acid, quercetin, myricetin, quercetin-3-O-glucuronide, 4-methoxyquerceting, p-coumaric acid, epicatechin, kaempferol, and quercetin-3-O-rutinoside as well as sodium carbonate and Folin and Ciocalteu’s reagent (2N) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Extraction and LC-MS grade solvents, including methanol, water, acetonitrile, hexanes, and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). VitaBlue Pure American Blueberry Extract was obtained from FutureCeuticals (Momence, IL, USA); lyophilized composite wild blueberry (Vaccinium angustifolium) powder was obtained from the Wild Blueberry Association of North America (Old Towne, ME, USA).
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