Log In Sign up
The largest database of trusted experimental protocols

Anti cd86

Manufactured by BioXCell
Visit Supplier

Anti-CD86 is a lab reagent used to detect and quantify the expression of the CD86 protein, which is involved in the activation of T cells. It can be used in various immunological and cell biology applications, such as flow cytometry and cell sorting.

Automatically generated - may contain errors

Lab products found in correlation

Magnetic separation, by Miltenyi Biotec (3 mentions) Anti cd3, by BD (2 mentions) Il 21, by Thermo Fisher Scientific (1 mentions) Il 21, by Miltenyi Biotec (1 mentions) Ctla4 ig, by Bristol-Myers Squibb (1 mentions) Interleukin 7 (il 7), by BioXCell (1 mentions) Anti ctla4, by BioXCell (1 mentions) Cd40l, by BioXCell (1 mentions) Mouse igg2a, by BioXCell (1 mentions) Anti icos, by BioXCell (1 mentions) Ifn γ, by BioXCell (1 mentions) Anti pd 1, by BioXCell (1 mentions) Mouse igg1, by BioXCell (1 mentions) Rat igg2a, by BioXCell (1 mentions)

Spelling variants of this product

Anti-CD86 (clone GL-1 (2 citations)

4 protocols using anti cd86

1

Investigating CD4+ T Cell Response in IL-21R-/- Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD19+ B cells from BALB/c or IL-21R−/− spleen were isolated by magnetic separation (Miltenyi Biotec) and cultured for 16 h with 1 μg/ml OVA peptide. Peptide-pulsed cells (3 × 106) were injected i.v. into IL-21R−/− recipients. magnetic separation (Miltenyi Biotec) was used to purify CD4+ cells from IL-21R−/− DO11.10 TCR+ lymph node. Twenty-four hours after B cell transfer, a total of 2 × 106 CellTrace Violet–labeled IL-21R−/− DO11.10 TCR+ T cells were injected i.v. and 1 μg IL-21 (PeproTech) or PBS i.p. Where indicated recipients were also given 100 μg anti-CD86 (Bio X Cell) or PBS i.p. immediately after T cell transfer. Mice were culled at day 7 for analysis of inguinal lymph node and splenic cells.
Attridge K., Kenefeck R., Wardzinski L., Qureshi O.S., Wang C.J., Manzotti C., Okkenhaug K, & Walker L.S. (2014). IL-21 Promotes CD4 T Cell Responses by Phosphatidylinositol 3-Kinase–Dependent Upregulation of CD86 on B Cells. The Journal of Immunology Author Choice, 192(5), 2195-2201.
+ Open protocol
+ Expand
2

CD4+CD25- T cell activation assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Magnetic separation (Miltenyi Biotec) was used to purify CD4+CD25− T cells from IL-21R−/− lymph node. 2.5×104 cells were cultured with 2.5×104 CD19+ B cells from BALB/c or IL-21R−/− spleen, with 0.8μg/ml anti-CD3 (BD Biosciences) alone or in the presence of 10μg/ml anti-CD86 (BioXcell). Triplicate wells were pooled and harvested at day 1 to assess CD86 expression or day 3 to determine cell counts by flow cytometry.
Attridge K., Kenefeck R., Wardzinski L., Qureshi O.S., Wang C.J., Manzotti C., Okkenhaug K, & Walker L.S. (2014). IL-21 Promotes CD4 T Cell Responses by Phosphatidylinositol 3-Kinase–Dependent Upregulation of CD86 on B Cells. The Journal of Immunology Author Choice, 192(5), 2195-2201.
+ Open protocol
+ Expand
3

Antibody Modulation of Immune Checkpoints

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 mice were injected with following mAbs as indicated in figure legends: 250 μg/dose mouse IgG1 (Clone: MOPC-21; BioXcell), mouse IgG2a (Clone: C1.18.4), Rat-IgG2a (Clone: 2A3; BioXcell), of human CD28 domain specific antibody (100 μg/dose; Bristol-Myers Squibb), 250 μg/dose of anti-CD80 (Clone: 1G10; BioXcell), anti-CD86 (Clone: GL-1; BioXcell) CTLA4-Ig (Abatacept, Bristol-Myers Squibb), Anti-ICOS (Clone: 7E.17G9; BioXcell), Anti-CTLA4 (Clone UC10-4F10-11; Bio X Cell); Anti-PD1(Clone: 29F.1A12; BioXcell), TIM-3(Clone: RMT3.23; BioXcell), TIGIT (Clone:1G9; BioXcell), BTLA (Clone:6A6, BioXcell), VISTA(Clone: 13F3; BioXcell), CD70(Clone: FR70; BioXcell), CD48(Clone: HM48.1; BioXcell), TNF-α (Clone: XT3.11; BioXcell), IFNγ (Clone: XMG1.2; BioXcell), IFNAR (Clone:MAR1–5A3; BioXcell), IFNγR (Clone:GR20), IL-7 (Clone:M25; BioXcell), IL-7Rα (Clone:A7R34; BioXcell), CD40 (50 μg/dose; Clone:FGK4.5; BioXcell, CD40L (MR-1; BioXcell) Clone:, GITR (Clone:DTA-1; BioXcell), OX40 (Clone:OX-86; BioXcell), 4-1BB (Clone:LOB12.3; BioXcell), and Anti-MHC-I (500 μg/dose; Clone: M1/42; BioXcell).
Panda A.K., Kim Y, & Shevach E.M. (2022). Control of Memory Phenotype T Lymphocyte Homeostasis: Role of Costimulation. Journal of immunology (Baltimore, Md. : 1950), 208(4), 851-860.
+ Open protocol
+ Expand
4

T Cell-B Cell Co-Culture Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Magnetic separation (Miltenyi Biotec) was used to purify CD4+CD25− T cells from IL-21R−/− lymph node. Cells (2.5 × 104) were cultured with 2.5 × 104 CD19+ B cells from BALB/c or IL-21R−/− spleen, with 0.8 μg/ml anti-CD3 (BD Biosciences) alone or in the presence of 10 μg/ml anti-CD86 (Bio X Cell). Triplicate wells were pooled and harvested at day 1 to assess CD86 expression or day 3 to determine cell counts by flow cytometry.
Attridge K., Kenefeck R., Wardzinski L., Qureshi O.S., Wang C.J., Manzotti C., Okkenhaug K, & Walker L.S. (2014). IL-21 Promotes CD4 T Cell Responses by Phosphatidylinositol 3-Kinase–Dependent Upregulation of CD86 on B Cells. The Journal of Immunology Author Choice, 192(5), 2195-2201.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!