Mbt 7854 msp library

Information about the different strains used in this study is provided in Data Set S1 in the supplemental material. Samples were either collected in the framework of routine diagnostic procedures or as part of investigations on a permit issued by the accredited Institutional Animal Care and Use Committee of the International Livestock Research Institute in Nairobi, Kenya (approval no. IACUC 2014.8). The Staphylococcaceae strains were isolated using standard methods (37 ) without any enrichment to select for high MICs and stored for subsequent use at −80°C. Species designation of the various strains was first performed via MALDI-TOF MS analysis and then, once the draft genomes were obtained (see below), confirmed by genomic sequence analysis using the type strain genome server (TYGS) (18 (link)). For MALDI-TOF MS analysis, strains were streaked onto trypticase soy agar with 5% sheep blood (TSA-B; Becton, Dickinson and Co.) and incubated at 37°C overnight. A small amount of colony material was transferred onto a steel target plate, 1 μL of 70% formic acid was added to the colony material, and it was allowed to air dry before 1 μL of HCCA matrix solution (α-cyano-4-hydroxycinnamic acid; Bruker Daltonics) was added. MALDI-TOF MS measurements to identify the species were performed using a Microflex LT instrument (Bruker Daltonics) and the MBT 7854 MSP Library.