Anti mir 298

The cell transfections were performed using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instructions. siRNA against STAT3 (Ruibio, Guangzhou, China) and a non-targeting siRNA control (Ruibio, Guangzhou, China) were used to knock down gene expression. Briefly, the oligonucleotides and plasmids were transfected using Lipofectamine 2000 (Invitrogen) according the manufacturer’s protocol. 48 h later, G418 was added into the medium to select of stable clones. The pcDNA3.1-STAT3 and pcDNA3.1 vectors were purchased from Santa Cruz Biotechnology (USA). miR-298, miR-ctrl, anti-miR-ctrl and anti-miR-298 were purchased from Genechem (Shanghai, China).
The shRNA sequence that targeted LINC01287 was AAGCATTGTAGACCTGGCTGCTGAA. The sequences were cloned into the pGFP-C-shLenti vector according to the manufacturer’s instructions (Origene). Then, the viruses were packaged in 293 T cells according to a standard protocol. HCC cells were infected with virus particles plus 6 μg/ml Polybrene.

The cell transfections were carried out using lipofectamine 2000 reagent (Invitrogen, Carlsbad, USA) according to manufactures instructions. siRNA against MYB (Ruibio, Guangzhou, China) and a non‐targeting siRNA control (Ruibio) were used to knockdown gene expression. The vector pcDNA3.1‐MYB and pcDNA3.1 were purchased from Santa Cruz (Santa Cruz, CA, USA). miR‐298, miR‐ctrl, anti‐miR‐ctrl and anti‐miR‐298 were purchased from Genechem (Shanghai, China).
shRNA sequences targeting LINC01287 were AAGCATTGTAGACCTGGCTGCTGAA. The sequences were cloned into the pGFP‐C‐shLenti vector according to manufacturer's instructions (Origene, Rockville, MD, USA). Then, the viruses were packaged using 293T cells according to standard protocol. HCC cells were infected with virus particles plus 6 μg/mL Polybrene (Sigma‐Aldrich, St. Louis, MO, USA).