Cenp e

Manufactured by Merck Group

CENP-E is a mitotic kinesin-like protein that plays a crucial role in the proper segregation of chromosomes during cell division. It is responsible for the alignment of chromosomes on the mitotic spindle and the subsequent movement of chromosomes to the poles during anaphase.

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Lab products found in correlation

Actin 1 19, by Santa Cruz Biotechnology (1 mentions) Pericentrin, by Abcam (1 mentions) α tubulin b 5 1 2, by Merck Group (1 mentions) Bubr1, by Fortis Life Sciences (1 mentions) Clip170, by Santa Cruz Biotechnology (1 mentions) Zwint1, by Fortis Life Sciences (1 mentions) Dynein, by Merck Group (1 mentions) Cenpf, by Abcam (1 mentions) Pierce co immunoprecipitation kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Rabbit anti-CENP-E (3 citations)

2 protocols using cenp e

Comprehensive Mitotic Spindle Protein Analysis

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The following antibodies were commercially purchased and used at the indicated dilutions for immunofluorescence (IF) and immunoblotting (IB); Actin (I-19, Santa Cruz Biotechnology, IB; 1/2000), α-tubulin (B-5-1-2, Sigma, IF; 1/1000), BubR1 (Bethyl Laboratories, IF; 1/500), CENP-E (Sigma, IF; 1/500, IB; 1/2000), CENP-F (Abcam, IF; 1/1000), CLIP170 (Santa Cruz Biotechnology, IF; 1/500), dynein intermediate chain (DIC, Sigma, IF; 1/500), Hec1 (9G3, Abcam, IF; 1/1000, IB; 1/2000), Kid (Cytoskeleton Inc., IB: 1/1000), KNL1 (Novus Biologicals, IF; 1/500), Mad2 (Novus Biologicals, IF; 1/500), Nde1 (Protein Tech Group, IF; 1/500), Ska1 (Abcam, IF; 1/500), Spindly (Bethyl Laboratories, IF; 1/500, IB; 1/2000), Zwint-1 (Bethyl Laboratories, IF; 1/500), Zw10 (Cosmo Bio, IF; 1/200, IB; 1/2000), pericentrin (Abcam, IF; 1/500).

Itoh G., Ikeda M., Iemura K., Amin M.A., Kuriyama S., Tanaka M., Mizuno N., Osakada H., Haraguchi T, & Tanaka K. (2018). Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment. Scientific Reports, 8, 3888.

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Protein Co-Immunoprecipitation Assay

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The co-immunoprecipitation of proteins was performed using a Pierce Co-Immunoprecipitation Kit (Thermo Fisher Scientific). The rabbit KIFC1 and CENPE antibodies (Sigma-Aldrich) and negative control rabbit IgG antibody were used in ASVEC. The complexes after immunoprecipitation were subjected to western blotting for detecting mutual binding between KIFC1 and CENPE.

Li J., Diao H., Guan X, & Tian X. (2020). Kinesin Family Member C1 (KIFC1) Regulated by Centrosome Protein E (CENPE) Promotes Proliferation, Migration, and Epithelial-Mesenchymal Transition of Ovarian Cancer. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e927869-1-e927869-10.

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