The KBD and its three components as follows: (i) Nelumbo nucifera petals (NN), (ii) Piper nigrum fruits (BP), and (iii) the aerial part of Centella asiatica (CA) were provided by Chao Phya Abhaibhubejhr Hospital, Prachinburi Province, Thailand. The plants were identified by Benjawan Leenin, a chief of Traditional Knowledge Center, Chao Phya Abhaibhubejhr Hospital Foundation. The relative herbarium voucher specimens were deposited at the museum of Chao Phya Abhaibhubejhr Hospital with following voucher numbers: ABH15, ABH18, and ABH17, respectively. Acetylthiocholine iodide (ATCI), bovine serum albumin (BSA), beta amyloid 1-42 (Aβ1–42), gallic acid, quercetin, β carotenoid, tacrine, trolox, N-acetyl cysteine (NAC), trypsine, fetal bovine serum (FBS), and Dulbecco's modified Eagle medium nutrient mixture F-12 (DMEM/F12) were purchased from Sigma-Aldrich (SM Chemical supplies Co., Ltd., Bangkok, Thailand), Merck (Merck, Bangkok, Thailand), Gibthai (GT Chemical supplies Co., Ltd., bangkok, Thailand), and Fluka (SM Chemical supplies Co., Ltd. ,Bangkok, Thailand).
β carotenoid
Manufactured by Merck Group
Sourced in Thailand, United States
β-carotenoid is a naturally occurring organic compound that functions as a precursor to vitamin A. It is a member of the carotenoid family of pigments. β-carotenoid can be extracted and utilized as a laboratory reagent.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Merck Group (2 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Acetylthiocholine iodide, by Merck Group (1 mentions)
Tacrine, by Merck Group (1 mentions)
Trypsine, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f12, by Merck Group (1 mentions)
Folin ciocalteu phenol reagent, by MP Biomedicals (1 mentions)
3 protocols using β carotenoid
1
Multicomponent Herbal Blend for Neuroprotection
2
HPLC Analysis of Carotenoids in Fruits
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Carotenoids were extracted from fruits and analyzed by HPLC, according to a method previously described by Xiong [33] . Component's identification and quantitative analysis were done using Agilent 1200 HPLC-DAD analysis system, 5 μm C18 m reverse phase column (250 mm × 4.6 mm, Japan) and 20 mm × 4.6 mm C18 pre column maintained at 35 °C. Using external standard method. The compounds β-carotenoid, and lutein were detected at different stages. The standard samples of β-carotenoid and lutein were purchased from Sigma-Aldrich Company (Ge). The concentration of β-carotenoid and lutein were calculated from the experimental peak area by analytical interpolation in a standard calibration curve and was expressed as mg 100 g -1 of fresh weight.
3
Antioxidant Assays of Citrus Fiber
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Folin-Ciocalteu phenol reagent was purchased from MP Biomedicals, LLC (Santa Ana, CA, USA). Aluminium chloride anhydrous (AlCl 3 ), potassium acetate (CH 3 COOK), and sodium carbonate (Na 2 CO 3 ) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Quercetin and β-carotenoid were purchased from Sigma-Aldrich® Co. (St. Louis, MO, USA). Gallic acid was purchased from Fluka® Analytical (St. Louis, MO, USA). An ORAC antioxidant assay kit was purchased from Zen-Bio, Inc. (Research Triangle Park, NC, USA). Citrus fibre (CitraFiber™), which is a fine powder, was provided by the Natural Citrus Products Corporation (Fort Pierce, FL, USA).
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