HCCLM3 (RRID:CVCL_6832, catalog no. GDC214) was obtained from China Center for Type Culture Collection, and Huh7 (RRID:CVCL_0336, catalog no. TCHu182) cell lines were purchased from the Council Cell Bank of Typical Culture Preservation. Rabbit anti-p-MEK polyclonal antibody (RRID:AB_331648, catalog no. 9121), mouse anti-ERK monoclonal antibody (RRID:AB_390780, catalog no. 4696), rabbit anti-p-ERK polyclonal antibody (RRID:AB_2315112, catalog no. 4370), and rabbit anti-GAPDH monoclonal antibody (RRID:AB_10622025, catalog no. 5174) were purchased from Cell Signal Technology. Rabbit anti-tubulin polyclonal antibody (RRID:AB_10575456, catalog no. MBS530753) was obtained from MyBiosource. Rabbit anti-AZGP1 polyclonal antibody (RRID:AB_11159784, catalog no. ab117275); rabbit anti-cyclin D1 monoclonal antibody (RRID:AB_2750906, catalog no. ab134175); rabbit anti-CDK4 monoclonal antibody (RRID:AB_10867218, catalog no. ab108357); rabbit anti-histone polyclonal antibody, nuclear loading control, and ChIP grade (RRID:AB_302613, catalog no. ab1791); rabbit anti-Ki-67 polyclonal antibody (RRID:AB_443209, catalog no. ab15580); goat anti-mouse immunoglobulin G (IgG) polyclonal antibody (RRID:AB_954556; catalog no. ab205718); and goat anti-rabbit IgG polyclonal antibody (RRID:AB_955447, catalog no. ab6721) were purchased from Abcam (USA).
Goat anti rabbit igg polyclonal antibody
Manufactured by Abcam
Sourced in United States
Goat anti-rabbit IgG polyclonal antibody is a secondary antibody that binds to rabbit immunoglobulin G (IgG) antibodies. It is used to detect and quantify rabbit primary antibodies in various immunoassays and applications.
Automatically generated - may contain errors
Lab products found in correlation
Cell mitochondria isolation kit, by Beyotime (2 mentions)
Goat anti mouse igg polyclonal antibody, by Abcam (2 mentions)
Hcclm3, by National Collection of Authenticated Cell Cultures (1 mentions)
Rabbit monoclonal anti gapdh antibody, by Cell Signaling Technology (1 mentions)
Rabbit polyclonal anti ki67 antibody, by Abcam (1 mentions)
Ecl detection system, by Thermo Fisher Scientific (1 mentions)
Ab53100, by Abcam (1 mentions)
Ab64715, by Abcam (1 mentions)
Ab52915, by Abcam (1 mentions)
Ab52631, by Abcam (1 mentions)
Ab21610, by Abcam (1 mentions)
Ab40855, by Abcam (1 mentions)
Ab8245, by Abcam (1 mentions)
Ab5694, by Abcam (1 mentions)
E cadherin, by Affinity Biosciences (1 mentions)
Ab32064, by Abcam (1 mentions)
Ab50887, by Abcam (1 mentions)
Ab196495, by Abcam (1 mentions)
Ab180905, by Abcam (1 mentions)
Ab220163, by Abcam (1 mentions)
3 protocols using goat anti rabbit igg polyclonal antibody
1
Antibody Characterization for Cell Signaling
2
Mitochondrial Protein Extraction and Western Blot Analysis
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Using a Cell Mitochondria Isolation Kit (Beyotime, Shanghai, China), we extracted proteins from target cells. The samples were incubated with the following primary antibodies: anti-TGFβ1 (ab21610, Abcam, Cambridge, MA, USA), anti-TGFβ1 (ab64715, Abcam), anti-α-SMA (ab5694, Abcam), anti-p-SMAD2 (ab53100, Abcam), anti-SMAD2 (ab40855, Abcam), anti-MMP1 (ab52631, Abcam), anti-MMP3 (ab52915, Abcam), anti-ACTG2 (SAB1411364, Sigma-Aldrich, St. Louis, MO, USA), and anti-GAPDH (ab8245, Abcam) overnight at 4°C; afterward, the samples were incubated with goat anti-rabbit IgG polyclonal antibody (Abcam) or goat anti-mouse IgG polyclonal antibody (Abcam) for 1 h at room temperature. Visualization was conducted using an enhanced chemiluminescence (ECL) detection system (Thermo Fisher Scientific) using GAPDH levels as an endogenous control.
3
Mitochondrial Protein Profiling for Cell Signaling
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Total proteins were collected from target cells with a Cell Mitochondria Isolation Kit (Beyotime, Shanghai, China). The samples were incubated at 4 °C for one night with the primary antibodies METTL3 (15073-1-AP, Proteintech), Bcl-2 (ab196495, Abcam), Bax (50599-2-Ig, Proteintech), Caspase 9 (10380-1-AP, Proteintech), Caspase 3 (19677-1-AP, Proteintech), cleaved PARP-1 (ab32064, Abcam), E-cadherin (BF0219, Affinity), N-cadherin (AF4039, Affinity), vimentin (10366-1-AP, Proteintech), PTEN (22034-1-AP, Proteintech), YTHDF2 (ab220163, Abcam), p53 (CSB-PA07889A0Rb, CUSABIO Life Sciences, College Park, USA), c-Myc (10057-1-AP, Proteintech), NF-κb (ab76302, Abcam), Zeb1 (ab180905, Abcam), Zeb2 (ab191364, Abcam), Snail (ab180714, Abcam), Twist1 (ab50887, Abcam); after that, the samples were incubated at room temperature for 1 h with goat anti-rabbit IgG polyclonal antibody (Abcam) or goat anti-mouse IgG polyclonal antibody (Abcam). GAPDH levels were used as an endogenous control for visualization employing the enhanced chemiluminescence (ECL) detection method (Thermo Fisher Scientific).
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