Ryngo 1 23

Wortmannin (Selleck, Houston, TX, USA) was diluted upon receipt to 40mM stock concentration in DMSO, Dynasore (Cayman Chemical, Ann Arbor, MI, USA) and EHop-016 (Cayman) were diluted to 25mM stock concentration in DMSO, and Ryngo 1–23 (Abcam, Cambridge, MA, USA) was diluted to 20mM stock concentration in DMSO. All inhibitors were dispensed into single use aliquots and stored at −20°C for no longer than 1 year after receipt. Wortmannin was diluted to a working concentration of 40nM (1:10000), Dynasore was diluted to a working concentration of 25 μM (1:1000), EHop-016 was diluted to a working concentration of 10μM (1:2500), and Ryngo 1–23 was diluted to a working concentration of 40μM (1:500), each using supplemented DMEM as diluent.

Dyngo 4a (ab120689) and Ryngo 1-23 (ab146050) were both purchased from Abcam. Human Tf Alexa Fluor 647-conjugate (T23366) was bought from Invitrogen and SiR-actin (SC0001) from Spirochrome. 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-3-trimethylammonium-propane (chloride salt; DOTAP), and TopFluor-cholesterol (Bodipy-Chol) was purchased from Avanti Polar Lipids Inc (Alabaster). DMSO (34943), chloroform (CHCl₃), and methanol (MeOH) were purchased from Sigma-Aldrich. Rat brain (substansia nigra) protein lysate was a kind gift from Roine El-Habta, Umeå University, Umeå, Sweden.