Hyperreztm xp carbohydrate h 8 μm column

Residual sugars (glucose and fructose), glycerol, ethanol and acetic acid from the fermentation end point samples were determined by HPLC (Thermo Fisher Scientific, Waltham, MA. USA) using a refraction index detector and a HyperREZTM XP Carbohydrate H+ 8 μm column (Thermo Fisher Scientific) equipped with a HyperREZTM XP Carbohydrate Guard (Thermo Fisher Scientific). Samples were diluted 3-fold, filtered through a 0.22-μm nylon filter (Symta, Madrid, Spain) and injected in duplicate. The analysis conditions were: eluent, 1.5 mM of H₂SO₄; 0.6 ml min-1 flux and a 50°C oven temperature.

Residual sugars (glucose and fructose), glycerol, ethanol and acetic acid from the fermentation end point samples were determined by HPLC (Thermo Fisher Scientific, Waltham, MA. USA) using a refraction index detector and a HyperREZTM XP Carbohydrate H+ 8μm column (Thermo Fisher Scientific) equipped with a HyperREZTM XP Carbohydrate Guard (Thermo Fisher Scientific). Samples were diluted to maintain our target compounds within the allowed range of detection, filtered through a 0.22 μM nylon filter (Symta, Madrid, Spain) and injected in duplicate. The analysis conditions were: eluent, 1.5 μM of H₂SO₄; 0.6 mL/min flux and a 50°C oven temperature.

Sugars and organic acids were extracted and determined essentially as described in Zacarías-García et al. [31 (link)]. Sugars and organic acids content was measured by HPLC (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a refraction index detector. For glucose, fructose, citric acid, malic acid, quinic acid, and succinic acid, separation and determination were employed in a HyperREZTM XP Carbohydrate H+ 8 μm column (Thermo Fisher Scientific). For sucrose determination, a Pb column was used (Hi-Plex Pb, 300 × 7.7 mm, Agilent Technologies). Chromatographic conditions used are described in Pérez-Través et al. [48 (link)]. The identification and quantification of sugars and organic acids were carried out by comparison with the retention times of authentic standards (Sigma-Aldrich, Barcelona, Spain). Two independent replicates of each sample were analyzed, and the results are expressed as mean ± standard deviation.