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2 protocols using tetrahydrocurcumin

1

Tetrahydrocurcumin Modulates Epithelial-Mesenchymal Transition

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Tetrahydrocurcumin was purchased from Yuanye Biotechnology Co. Ltd (Shanghai,China). E-cadherin, ZEB, Snail, Twist, HIF-1α, p-mTOR, mTOR, p-ERK, ERK, GAPDH and β-actin antibodies were purchased from Santa cruz Biotechnology (Santa cruz, CA, USA). Vimentin, N-cadherin, VEGF, MMP-2, MMP-9, LaminA/C, Atg5, Atg7 and Beclin-1 antibodies were obtained from Boster Biological Technology Co. Ltd (Wuhan, China). LY294002, SB203580, Rapamycin, β-catenin, p-Akt, Akt, p-JNK, JNK, p-p38, p38, IκB-α, p65, Hsp90 and GSK-3β antibodies were obtained from Beyotime Institute of Biotechnology (Haimen, China). Antibody against LC3B was purchased from Abcam (Cambridge, UK). All other reagents were from common commercial sources.
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2

Curcuminoid and Oseltamivir Carboxylate Acquisition

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Dihydrocurcumin, tetramethylcurcumin, bisdemethoxycurcumin, curcumin-d6, demethoxycurcumin, demethylcurcumin, dimethylcurcumin, curcumin, tetrahydrocurcumin, octahydrocurcumin, hexahydrocurcumin and oseltamivir carboxylate were acquired from Shanghai yuanye Bio-Technology Co. Ltd. (Shanghai, China). Purity was ≥ 99% by high-performance liquid chromatography (HPLC).
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