Log In Sign up
The largest database of trusted experimental protocols

Rho associated kinase rock inhibitor

Manufactured by Merck Group
Visit Supplier

Rho-associated kinase (ROCK) inhibitor is a type of lab equipment used in research and development. It functions by inhibiting the activity of the ROCK enzyme, which plays a role in various cellular processes. The ROCK inhibitor is commonly utilized in experiments involving cell signaling, cytoskeleton dynamics, and other areas of cell biology research.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions) Geltrex, by Thermo Fisher Scientific (2 mentions) Normocin, by InvivoGen (2 mentions) Withaccutase, by STEMCELL (2 mentions)

Spelling variants of this product

ROCK inhibitor (75 citations) Rho kinase inhibitor (7 citations)

2 protocols using rho associated kinase rock inhibitor

1

Culturing Human Embryonic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human Embryonic Stem Cell (hESC) line (HUES66) was obtained from the
Harvard Stem Cell Institute. Stem cells were cultured in 10 cm tissue culture
dishes coated with 5 ml of geltrex (Thermo-Fisher Scientific) diluted in DMEM
(Thermo-Fisher Scientific) with 1:100 ratio for 30 min at 37 °C. Stem
cell culture medium contained mTeSR (Stemcell Technologies) with provided
supplement and normocin (InvivoGen). Stem cells were passaged by detaching with
accutase (Stemcell Technologies) diluted in DPBS (Thermo-Fisher Scientific) at
1:3. After each splitting of stem cells, 10μM Rho-associated kinase
(ROCK) inhibitor (EMD Millipore) was added in medium of re-plated cells. Stem
cell culture medium was replaced daily.
Tekin H., Simmons S., Cummings B., Gao L., Adiconis X., Hession C.C., Ghoshal A., Dionne D., Choudhury S.R., Yesilyurt V., Sanjana N.E., Shi X., Lu C., Heidenreich M., Pan J.Q., Levin J.Z, & Zhang F. (2018). Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature biomedical engineering, 2(7), 540-554.
+ Open protocol
+ Expand
2

Culturing Human Embryonic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human Embryonic Stem Cell (hESC) line (HUES66) was obtained from the
Harvard Stem Cell Institute. Stem cells were cultured in 10 cm tissue culture
dishes coated with 5 ml of geltrex (Thermo-Fisher Scientific) diluted in DMEM
(Thermo-Fisher Scientific) with 1:100 ratio for 30 min at 37 °C. Stem
cell culture medium contained mTeSR (Stemcell Technologies) with provided
supplement and normocin (InvivoGen). Stem cells were passaged by detaching with
accutase (Stemcell Technologies) diluted in DPBS (Thermo-Fisher Scientific) at
1:3. After each splitting of stem cells, 10μM Rho-associated kinase
(ROCK) inhibitor (EMD Millipore) was added in medium of re-plated cells. Stem
cell culture medium was replaced daily.
Tekin H., Simmons S., Cummings B., Gao L., Adiconis X., Hession C.C., Ghoshal A., Dionne D., Choudhury S.R., Yesilyurt V., Sanjana N.E., Shi X., Lu C., Heidenreich M., Pan J.Q., Levin J.Z, & Zhang F. (2018). Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature biomedical engineering, 2(7), 540-554.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!