Stock solutions of
the test compounds
were prepared in DMSO (Fisher Scientific) and diluted in Milli-Q water.
A 400 ng portion of pUC19 dsDNA (2686 bp) plasmid (New England BioLabs)
was incubated with 50 μM of the test compounds or cisplatin
for different time intervals (15 min to 6 h) at 37 °C under continuous
shaking. In addition to the untreated control, a linear pUC19L vector
(ThermoFisher Scientific) was used. A 20 μL portion of the samples
was added to 4 μL of 6× DNA loading dye (ThermoFisher Scientific)
and loaded into the pockets of 1% agarose gel in 1× TBE buffer.
Electrophoresis was carried out at 60 V for 5 min, followed by 120
V for 90 min. Ethidium bromide (SERVA) staining was performed in 1×
TBE (0.75 μg/mL) for 20 min. Images were taken by the GelDoc-It
Imaging System Fusion Fx7 (Vilber Lourmat, Germany). For quantification
of the spots, ImageJ/Fiji1.46 was used.
the test compounds
were prepared in DMSO (Fisher Scientific) and diluted in Milli-Q water.
A 400 ng portion of pUC19 dsDNA (2686 bp) plasmid (New England BioLabs)
was incubated with 50 μM of the test compounds or cisplatin
for different time intervals (15 min to 6 h) at 37 °C under continuous
shaking. In addition to the untreated control, a linear pUC19L vector
(ThermoFisher Scientific) was used. A 20 μL portion of the samples
was added to 4 μL of 6× DNA loading dye (ThermoFisher Scientific)
and loaded into the pockets of 1% agarose gel in 1× TBE buffer.
Electrophoresis was carried out at 60 V for 5 min, followed by 120
V for 90 min. Ethidium bromide (SERVA) staining was performed in 1×
TBE (0.75 μg/mL) for 20 min. Images were taken by the GelDoc-It
Imaging System Fusion Fx7 (Vilber Lourmat, Germany). For quantification
of the spots, ImageJ/Fiji1.46 was used.