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2 protocols using hispidulin

1

Hispidulin-Based Pain Management Protocol

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Hispidulin (ChemFaces, Wuhan, China), tween 80 (Srichansahasoth Co., Thailand), acetic acid (Merck, Darmstadt, Germany), formaldehyde (Merck), indomethacin (IND; Sigma), morphine sulfate (MRP; Thai FDA), and sterile water (SW; General Hospital Products Public Co.) were used in this study. HPLC grade acetonitrile and methanol (MeOH) were purchased from RCI Labscan (Bangkok, Thailand). Ultrapure water was obtained from Barnstead™ MicroPure™ Water Purification System (Thermo Fisher Scientific, Massachusetts, USA). Glacial acetic acid was purchased from Merck KGaA (Darmstadt, Germany).
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2

Immune Checkpoint Inhibitor Screening Assays

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Caffeic acid (#CFN99190), luteolin (#CFN98784), luteolin 7-O-glucoside (#CFN98565), cosmosiin (#CFN98981), homoplantaginin (#CFN90344), apigenin (#CFN98843), and hispidulin (#CFN99491) were purchased from ChemFaces (Wuhan, China). PD-1/PD-L1 inhibitor screening assay kit (#72005), CTLA-4 (cytotoxic T-lymphocyte-associated protein 4)/CD80 inhibitor screening assay kit (#72009), and PD-L1-blocking antibody (#71213) were purchased from BPS Bioscience (San Diego, CA, USA). Small molecule PD-L1 inhibitor C1 (BMS-1, # T3655) was purchased from TargetMol (Wellesley Hills, MA, USA). Anti-PD-1 antibody (Pembrolizumab, #A2005) and anti-CTLA-4 antibody (#A2001) was purchased from Selleck Chemicals (Houston, TX, USA).
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