A431 cells (4 × 105/well) were seeded into a 6-well plate and transfected with WWP1 siRNA and control siRNA (consiRNA), respectively (both Santa Cruz Biotechnology). The cells were divided into the following three groups for transfection: A431 (untreated group), consiRNA (transfection with consiRNA) and WWP1 siRNA (transfection with WWP1 siRNA). Transfection was carried out with WWP1 siRNA and consiRNA at final concentrations of 100 µM using Lipofectamine 2000 (Invitrogen Corp., Carlsbad, CA, USA) according to the manufacturer’s instructions. The cells were then harvested and subjected to sqRT-PCR, western blotting, migration experiments, Transwell assays and flow cytometry at 48 hours after transfection.
Consirna
Manufactured by Santa Cruz Biotechnology
Sourced in United States
ConsiRNA is a laboratory tool designed for the analysis and study of gene expression. It utilizes a specific technology to facilitate the controlled and targeted silencing of gene expression in cell cultures and experimental models.
Automatically generated - may contain errors
Lab products found in correlation
Consirna, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Ampk sirna, by Santa Cruz Biotechnology (1 mentions)
Flag sirt1, by Addgene (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Sirt1 sirna, by Santa Cruz Biotechnology (1 mentions)
2 protocols using consirna
1
Knockdown of WWP1 in A431 Cells
2
SIRT1 and AMPK Regulation in VSMC
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VSMC was transfected with control siRNA or siRNA against SIRT1 and AMPK by using Lipofectamine 2000 Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. Con siRNA, SIRT1 siRNA, and AMPK siRNA were purchased from Santa Cruz (USA). Cells then were resuspended in complete DMEM, incubated for 24–48 hr, and used for further experiments. Flag-SIRT1 was obtained from Addgene plasmid repository (Addgene plasmid #1791; Greenberg ME, Harvard Medical School, Boston, MA, USA). For transfection, VSMC was seeded on 6-well plates and when 80% confluent, transfected in Opti-MEM/Lipofectamine 2000 Reagent containing SIRT1 plasmid DNA.
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