Rpmi 1640 culture media

A total of 20 clean laboratory-grade male Kunming mice aged 4 weeks old and 9 clean weaned piglets aged 40 days old were used in this study. The Laboratory Animal Center of Yanbian University provided the mice, and the piglets were obtained from a farm. The competent Escherichia coli BL21 cells were purchased from Sangong Bioengineering (Shanghai, China) and kept by the Preventive Veterinary Laboratory.
Restriction enzymes (Nde I and Xho I), T4 DNA ligase, and ConA were provided by TaKaRa. The Plasmid Mini I and Gel Extraction kits were provided by OMEGA. The cell proliferation and cytotoxicity detection kit (MIT), protein concentration determination kit (BCA), mouse spleen lymphocyte isolation kit, and porcine spleen lymphocyte isolation kit were obtained from Solarbio. HRP sheep anti-mouse IgG and HRP sheep anti-pig IgG were provided by SIGMA. Mouse anti-M. suis serum was prepared by the Preventive Veterinary Laboratory of Yanbian University. The IgG₁, IgG_2a, IFN-γ, and IL-4 kits were purchased from Sangon Biotech (Shanghai, China). The RPMI-1640 culture media and fetal bovine serum (FBS) were provided by Biological Industries (Kibbutz Beit-Haemek, Israel).

Female C57BL/6 (H-2^b) and BALB/C (H-2^d) mice were purchased from Shanghai Laboratory Animal Center (Shanghai, China). All mice were maintained in a specific pathogen-free room at Animal Facilities of Soochow University. Experiments were carried out and approved according to the guidelines of the animal care and use committee at Soochow University. A20 lymphoma cells were purchased from American Type Culture Collection (Rockville, MD, USA). Cells were cultured at 37°C in a 5% CO₂ incubator in RPMI 1640 culture media supplemented with 10% fetal bovine serum (Biological Industries, Co., Haemek, Israel). For bioluminescent imaging, stable luciferase-expressing A20 cells (A20-luc) were generated in our laboratory.