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Silicon nitride

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Silicon nitride is a type of thin film coating material used in various laboratory equipment. It serves as a protective and functional layer, providing characteristics such as hardness, wear resistance, and chemical stability. The core function of silicon nitride is to enhance the performance and longevity of the equipment in which it is applied.

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Lab products found in correlation

Cleaved mica, by SPI Supplies (2 mentions) Multimode afm, by Veeco (1 mentions) Nanoscope iiia controller, by Veeco (1 mentions)

2 protocols using silicon nitride

1

Multimode AFM Imaging of Pseudoviruses

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A Multimode™ AFM with a Nanoscope Ilia controller (Veeco, Santa Barbara, CA) operated in contact mode was used to image pseudoviruses before and after addition of vimentin or DNA. The cantilevers used were silicon nitride with a spring constant of 0.35 N/m (Veeco, Santa Barbara, CA). The AFM was calibrated using a 3D reference of 200 nm height and 10 μm pitch (Digital Instruments, Santa Barbara, CA). Deflection and height images were obtained with scan rates of 1 Hz with a resolution of 512 pixels/line. Briefly, for AFM evaluation of pseudoviruses in control (non-vimentin addition) and samples preincubated with human recombinant vimentin, a 10 μl drop of pseudoviruses or pseudoviruses/vimentin was applied on cleaved mica (SPI Supplies, West Chester, PA) and analyzed in a dried environment.
Suprewicz Ł., Swoger M., Gupta S., Piktel E., Byfield F.J., Iwamoto D.V., Germann D., Reszeć J., Marcińczyk N., Carroll R.J., Lenart M., Pyre K., Janmey P., Schwarz J.M., Bucki R, & Patteson A. (2021). Extracellular vimentin as a target against SARS-CoV-2 host cell invasion. bioRxiv.
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2

Atomic Force Microscopy of Pseudoviruses

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A Multimode ™ AFM with a Nanoscope IIIa controller (Veeco, Santa Barbara, CA) operated in contact mode was used to image pseudoviruses before and after addition of vimentin or DNA. The cantilevers used were silicon nitride with a spring constant of 0.35 N/m (Veeco, Santa Barbara, CA). The AFM was calibrated using a 3D reference of 200 nm height and 10 µm pitch (Digital Instruments, Santa Barbara, CA). Deflection and height images were obtained with scan rates of 1 Hz with a resolution of 512 pixels/line. Briefly, for AFM evaluation of pseudoviruses in control (non-vimentin addition) and samples preincubated with human recombinant vimentin, a 10 µl drop of pseudoviruses or pseudoviruses/vimentin was applied on cleaved mica (SPI Supplies, West Chester, PA) and analyzed in a dried environment.
Suprewicz Ł., Swoger M., Gupta S., Piktel E., Byfield F.J., Iwamoto D.V., Germann D., Reszeć J., Marcińczyk N., Carroll R.J., Janmey P.A., Schwarz J.M., Bucki R, & Patteson A.E. (2021). Extracellular vimentin as a target against SARS-CoV-2 host cell invasion. Small (Weinheim an der Bergstrasse, Germany), 18(6), e2105640.
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