Protein lysates from primary chondrocytes were prepared in 4× laemmli SDS sample buffer (Boston Bioproducts), denatured, separated in polyacrylamide gels 4%–20% (GeneScript), transferred on to nitrocellulose membranes, and subjected to immunoblot analysis using a Wnt11 antibody (Invitrogen # PA5-21712, 1:750) and a histone 3 antibody (Cell signaling #4499, 1:2000) according to a standard protocol. Signals were visualized by chemiluminescence (Thermo Fisher) and quantified on acquired images using ImageJ or the measurement function of Photoshop (Adobe ver.23.2).
Histone 3 antibody
Manufactured by Cell Signaling Technology
The Histone 3 antibody is a laboratory tool used to detect and quantify the presence of histone 3 protein in biological samples. Histone 3 is a core component of nucleosomes, the fundamental units of chromatin, and plays a crucial role in the regulation of gene expression and chromatin structure.
Automatically generated - may contain errors
2 protocols using histone 3 antibody
1
Chondrocyte Protein Expression Analysis
2
Molecular Profiling of Visceral Adipose and Liver
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Visceral fat tissues and liver tissues were collected and lysed in RIPA buffer. Nuclear protein was extracted from the liver tissues as previously described.18 (link) Western blot was performed as previously described.19 (link) β-actin was used as a loading control (Sigma-Aldrich, St. Louis, MO, USA). The primary antibodies including interleukin-6 (IL-6), the cell-bound precursor of tumor necrosis factor-α (TNF-α), adiponectin, nuclear factor-erythroid factor 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) were purchased from Abcam (Cambridge, MA). Histone-3 antibody was purchased from Cell Signaling Technology (Danvers, MA).
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