Human nestin

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Human nestin is a cytoskeletal protein that is expressed in neural stem and progenitor cells. It is commonly used as a marker for identifying and characterizing these cell types.

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Lab products found in correlation

Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Bradford assay, by Bio-Rad (1 mentions) Total akt, by Santa Cruz Biotechnology (1 mentions) Total erk, by Santa Cruz Biotechnology (1 mentions) Erbb2, by Santa Cruz Biotechnology (1 mentions) Erbb3, by Santa Cruz Biotechnology (1 mentions) Phospho akt s473, by Santa Cruz Biotechnology (1 mentions) Saponin, by Merck Group (1 mentions) Anti cd44, by Cell Signaling Technology (1 mentions) Ykl 40, by Quidel (1 mentions)

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Nestin (59 citations)

3 protocols using human nestin

Differentiation of Glial Stem Cells

Cited 1 time

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Differentiation of GSCs was induced by 7-day exposure to 5% fetal calf serum in DMEM. Staining for human nestin (Santa Cruz Biotechnology), GFAP (Sigma) and GalC (Chemicon) was performed as described previously ^{13 (link)}.

Jahan N., Lee J.M., Shah K, & Wakimoto H. (2017). Therapeutic targeting of chemoresistant and recurrent glioblastoma stem cells with a proapoptotic variant of oncolytic herpes simplex virus. International journal of cancer, 141(8), 1671-1681.

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Western Blot Analysis of Signaling Proteins

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Western blotting was performed as previously described.^{19 (link),30 (link)} Cell lysates were collected, and the protein concentration was determined by the Bradford assay (Bio-Rad, CA). The protein samples were electrophoresed on SDS-PAGE gels, transferred to PVDF membranes, and analyzed using antibodies against α-tubulin, phospho-AKT (S473), total-AKT, phospho-ERK (Thr202/Tyr204), total-ERK, phospho-STAT3 (Tyr705), STAT3, p21, SMARCA5 (Santa Cruz Biotechnology, USA), SMRT/NCOR2 (Santa Cruz Biotechnology, USA), ErbB3 (Santa Cruz Biotechnology, USA), ErbB2 (Santa Cruz Biotechnology, USA), human nestin (Santa Cruz Biotechnology, USA), and L1CAM (Fisher, USA). All antibodies were purchased from Cell Signaling, USA, unless otherwise indicated.

Alrfaei B.M., Clark P., Vemuganti R, & Kuo J.S. (2020). MicroRNA miR-100 Decreases Glioblastoma Growth by Targeting SMARCA5 and ErbB3 in Tumor-Initiating Cells. Technology in Cancer Research & Treatment, 19, 1533033820960748.

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Optimized IHC Protocol for CD44, Nestin, YKL-40

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Immunohistochemistry (IHC) for CD44 (Anti-CD44 from Cell Signaling Technology), human nestin (Santa Cruz) and YKL-40 (Quidel) was performed on formalin-fixed paraffin embedded sections using the protocol described previously^{7 (link)} with slight modifications. For antigen retrieval, 0.1% saponin (Sigma) treatment for 5 minutes was used for CD44 and YKL-40, and microwave in citrate buffer for human nestin.

Esaki S., Nigim F., Moon E., Luk S., Kiyokawa J., Curry W J.r., Cahill D.P., Chi A.S., Iafrate A.J., Martuza R.L., Rabkin S.D, & Wakimoto H. (2017). Blockade of transforming growth factor-β signaling enhances oncolytic herpes simplex virus efficacy in patient-derived recurrent glioblastoma models. International journal of cancer, 141(11), 2348-2358.

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