Anti p53 clone do 1

For immunoblotting and immunofluorescence assays, we used anti-p53 clone DO-1 (Santa Cruz Biotechnology, CA, USA), anti-histone H3 (Santa Cruz Biotechnology), anti-PROM1 (CD133) (Abnova, Taipei, Taiwan), anti-Nanog, anti-Oct4, and anti-Sox2 (Abcam, Cambridge, UK); anti-c-Myc (Santa Cruz Biotechnology); anti-EZH2 (BD Biosciences, Franklin Lakes, NJ, USA); and anti-Actin (Millipore, Billerica, MA, USA) primary antibodies. For ChIP assay, we used agarose conjugated with anti-HA (Santa Cruz Biotechnology); anti-p53, anti-HDAC1, anti-H3K9me3, and anti-H3 acetylation (Millipore) antibodies. For protein immunoprecipitation assays, anti-p53 (Santa Cruz Biotechnology) and anti-HDAC1 (Millipore) were used. For the BrdU assay, anti-BrdU (Abcam) was used. Dox was purchased from Sigma-Aldrich (St Louis, MO, USA).

Cell lysates were prepared and western blots were performed using the same procedures as previously described [18 (link)]. Antibodies used were anti-PKM (Santa-Cruz, clone C-11, 1 : 1000), anti-PGP (Santa-Cruz, clone E10, 1 : 1000), anti-β-actin (Sigma, clone AC-15, 1 : 5000), anti-p53 (clone DO1, Santa-Cruz, 1 : 1000), anti-phospho-p53 (Cell Signalling, 1 : 1000), anti-P-H2AX (Millipore, 1 : 1000) and anti-tubulin (Sigma, 1 : 40 000).