Anti ter119 biotin

Manufactured by BD

Anti-Ter119 biotin is a biotinylated monoclonal antibody that binds to the Ter119 antigen, which is expressed on red blood cells. This product is intended for use in flow cytometry and other immunological applications.

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Ter119 (80 citations) Anti-Ter119 (27 citations) Anti-Ter119 (TER119; (5 citations)

2 protocols using anti ter119 biotin

Multicolor Flow Cytometry for Immune Cell Profiling

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We used the following antibodies: anti-CD11b (M1/70) eFluor450 or PE; anti-CD11c (N418) AlexaFluor700; anti-human/mouse CD45R (B220) eFluor650NC; anti- CD59 and Gr1 (RB6-8C5) biotin; anti-CD199 (CCR9) PE/Cy7; anti-CD317 (ebio927) AlexaFluor488 or eFluor450; anti-MHCII (M5/114.15.2) FITC or PE/Cy5; anti-Sca1 (D7) biotin (eBioscience); anti-CD3 (145-2C11) biotin; anti-CD4 (RM4-5) biotin; anti-CD8 (53–6.7) biotin; anti-CD11b (M1/70) biotin; anti-CD45.1 (A20) PE; anti-human/mouse CD45R (B220) biotin; anti-CD115 (c-fms) APC; anti-CD135 (A2F10) PE; anti-CD172a (P84) APC; anti-NK1.1 (PK136) biotin; anti-Ter119 biotin (BD Biosciences); anti-CD11c (N418) biotin or APC; anti-CD19 (6D5) biotin; anti-CD45.1 (A20) FITC; anti-CD45.2 (104.2) PE or AlexaFluor700; anti-CD105 (Endoglin) Alexa488; anti-CD117 (c-kit) APC/Cy7; anti-Ly49Q (2E6) PE (MBL); anti-SiglecH (551.3D3) PE (BioLegend); AlexaFluor™ 405 (InvitroGen) or AlexaFluor488 Streptavidin (Jackson ImmunoResearch). Lineage staining was performed using a mixture of anti-CD3, -CD4, -CD8, -CD19, -CD11b, -CD11c, -Gr1, -Ter119, -NK1.1 and -B220 antibodies for Lin, and anti-CD3, -CD19, -Ter119, -NK1.1 and -B220 for Lin*. Cells were analyzed on a LSRII analyzer (BD Biosciences) and sorted on a FACSAriaIISORP (BD Biosciences). Sort purity was >98%.

Mastio J., Simand C., Cova G., Kastner P., Chan S, & Kirstetter P. (2018). Ikaros cooperates with Notch activation and antagonizes TGFβ signaling to promote pDC development. PLoS Genetics, 14(7), e1007485.

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Murine T cell subset isolation and sequencing

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Spleens were collected from euthanized mice and splenocytes were stained with anti-Ter-119-biotin (BD Biosciences), anti-B220-biotin (eBioscience), and anti-CD11c-biotin (eBioscience) antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C. B cells, erythrocytes, and monocytes were depleted on an AutoMACS separator (Miltenyi Biotec) following the manufacturer's procedure. Enriched T cells were stained for 20 min at 4°C with the following monoclonal antibodies at predetermined optimal dilutions: CD3-APC (BD Biosciences), CD4-Horizon-V500 (BD Biosciences), CD8-Alexa-700 (BD Biosciences), CD44-PE (BD Biosciences), and CD62L-eFluor-450 (eBioscience) and sorted on an FACSAria II cytometer (BD Biosciences) with a purity .95% into the following subsets: CD4 1 FoxP3 1 CD62 low CD44 high (amTregs), CD4 1 FoxP3 1 CD62 high CD44 low (nTregs), and CD4 1 FoxP3 2 (Teffs). A total of 1 3 10 5 -5 3 10 6 sorted cells were stored in RNAqueous kit lysis buffer (Invitrogen) at 280°C. cDNA Library Preparation for TR Sequencing RNA was extracted using the RNAqueous Total RNA Isolation Kit (Invitrogen). TRB libraries were prepared on 100 ng of RNA with the SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) and sequenced with HiSeq 2,500 single read (300 bp) (Illumina) plus 10% PhiX at the LIGAN Genomics platform (23) .

Mhanna V., Fourcade G., Barennes P., Quiniou V., Pham H.P., Ritvo P.G., Brimaud F., Gouritin B., Churlaud G., Six A., Mariotti-Ferrandiz E, & Klatzmann D. (2021). Impaired Activated/Memory Regulatory T Cell Clonal Expansion Instigates Diabetes in NOD Mice. Diabetes, 70(4).

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