Anti ctgf antibody

VP (cat. no. SML0534) was purchased from Sigma-Aldrich; Merck KGaA. The following primary antisera were used for the immunoblotting analysis: Anti-β-actin antibody (cat. no. 13E5; Cell Signaling Technology, Inc.), anti-YAP/TAZ antibody (cat. no. D24E4; Cell Signaling Technology, Inc), anti-Survivin antibody (cat. no. EP2880Y; Abcam), anti-CTGF antibody (cat. no. L-20; Santa Cruz Biotechnology, Inc.), and anti-CYR61/CCN1 antibody (cat. no. ab24448; Abcam). The following primary monoclonal antibodies were used for immunofluorescence: Anti-YAP antibody (cat. no. sc-101199; Santa Cruz Biotechnology, Inc.), anti-TAZ antibody (cat. no. ab84927; Abcam), and anti-CD31 antibody (cat. no. ab28364; Abcam). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (cat. no. ab6789; Abcam) and goat anti-rabbit IgG H&L (cat. no. ab97051; Abcam) were purchased for western blotting. Goat anti-mouse IgG H&L (Alexa Fluor^® 488; cat. no. ab150113; Abcam) was purchased for immunofluorescence.

DMEM was purchased from Gibco Invitrogen Corporation (Carlsbad, CA, USA). STZ was obtained from Sigma (Saint Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Biological Industries (BI, Beit HaEmek, Israel). Mouse and rat enzyme-linked immunosorbent assay (ELISA) kits were obtained from Elabscience Biotechnology (Wuhan, China). Antibodies were purchased from the following sources: anti-FN antibody, anti-TGF-β1 antibody, and anti-CTGF antibody were from Abcam (Cambridge, UK); anti-p-Akt1/2, anti-Akt1/2, anti-NF-κB p-p65, anti-p38, and anti p-p38 antibody were from Cell Signaling Technology, Inc. (Beverly, MA, USA); anti-α-SMA antibody was purchased from Gene Tex Inc. (Alton Parkway Irvine, CA, USA); and anti-α-tubulin monoclonal antibody was from Proteintech Group, Inc. (Chicago, USA). IRDye 680LT goat anti-mouse immunoglobulin G (IgG) and anti-rabbit IgG were obtained from LI-COR Biosciences (Lincoln, NE, USA).

For immunoblot analysis, 20 μg of whole‐liver lysate was resolved by TGX precast gels and transferred to polyvinylidene fluoride membranes (BioRad, Hercules, CA, USA). Blotted membranes were incubated with anti‐C1q antibody (Abcam) or anti‐CTGF antibody (Abcam), followed by peroxidase‐conjugated secondary antibody (GE Healthcare Life Sciences, Pittsburgh, PA, USA). Protein bands were visualized using enhanced chemiluminescence reagents (Thermo Fisher Scientific) and digitized using a charge‐coupled device camera (LAS4000 mini; Fuji film, Japan). Expression intensity was quantified by Multi Gauge (Fuji). Protein loading was verified using β‐actin (GeneTex) antibody.