Anti‐α‐tubulin (T9026 Sigma‐Aldrich Co.), anti‐neuron‐specific class III β‐tubulin (Tuj1, TUBB3 #80120, BioLegend) anti‐acetylated tubulin (T7451 Sigma‐Aldrich), anti‐GAPDH (MAB374 Merck Millipore) anti‐PKR (anti‐EIF2AK2 Antibody No. ABIN500507, antibodies‐online.com), anti‐V5 tag antibody (SV5‐Pk1, Abcam), anti‐tau antibody (in house produced polyclonal Tau antibody, affinity‐purified rabbit towards human 2N4R tau), anti‐tau (A0024, DAKO), anti‐STAT1 (#9172 Cell Signaling Technology), anti‐pSTAT1 (pS727, #9177 Cell Signaling Technology), anti‐pS199/202 tau (44‐768G Thermo Fisher Scientific), anti‐pS396 tau (44‐752G Thermo Fisher Scientific), AT8 (pS202/T205 tau, MN1020 Thermo Fisher Scientific), AT180 (pT231 tau, MN1040 Thermo Fisher Scientific), AT270 (pT181 tau, MN1050 Thermo Fisher Scientific), anti‐pS262 (44‐750G Thermo Fisher Scientific), anti‐pS404 (44‐758G Thermo Fisher Scientific), anti‐pS409 (Lu0041G, kindly provided by Lundbeck) and anti‐pS422 (ab79415, Abcam) were used in accordance with the manufacturers' recommendations. HRP‐conjugated rabbit immunoglobulins (#P0217, Dako) and secondary HRP conjugated mouse immunoglobulins (#P0260, Dako) were used as secondary antibodies.
Ab79415
Manufactured by Abcam
Ab79415 is a lab equipment product offered by Abcam. It is a technical specification without any interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Mn1060, by Thermo Fisher Scientific (2 mentions)
Anti stat1, by Cell Signaling Technology (1 mentions)
P0217, by Agilent Technologies (1 mentions)
Anti p stat1, by Cell Signaling Technology (1 mentions)
P0260, by Agilent Technologies (1 mentions)
Tubb3, by BioLegend (1 mentions)
A0024, by Agilent Technologies (1 mentions)
At180, by Thermo Fisher Scientific (1 mentions)
At270, by Thermo Fisher Scientific (1 mentions)
Sv5 pk1, by Abcam (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Mab348, by Merck Group (1 mentions)
Mn1020b, by Thermo Fisher Scientific (1 mentions)
Mab377, by Merck Group (1 mentions)
Mab374, by Merck Group (1 mentions)
Mn1020, by Thermo Fisher Scientific (1 mentions)
Af1828, by R&D Systems (1 mentions)
Trueblack solution, by Biotium (1 mentions)
Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions)
Prolong diamond antifade with dapi, by Thermo Fisher Scientific (1 mentions)
3 protocols using ab79415
1
Comprehensive Immunoblotting Protocol
2
Immunodetection of Tau Protein Phosphorylation
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To detect tau by immunostaining, we used biotin-conjugated anti-pS202/pT205 tau (AT8; 1:500; mouse monoclonal, catalog #MN1020B, Thermo Fisher Scientific) and anti-pT212/pS214/pT217 tau (AT100; 1:500; mouse monoclonal, MN1060, Thermo Fisher Scientific). The following additional antibodies were used for immunostaining: anti-Aβ (D12B2; 1:1000; rabbit monoclonal; catalog #9888, Cell Signaling Technology) and anti-NeuN (1:500; mouse monoclonal; catalog #MAB377, Millipore). For immunoblotting, we used the following antibodies: anti-mouse tau (T49; 1:50,000; mouse monoclonal; catalog #MABN827, Millipore), anti-APP (22C11; 1:2500; mouse monoclonal; catalog #MAB348, Millipore), anti-GAPDH (1:10,000; mouse monoclonal; catalog #MAB374, Millipore), AT8 (1:500), AT100 (1:500), anti-pS422 tau (1:1000; rabbit monoclonal; catalog #ab79415, abcam), anti-tau (BR133; 1:4000; rabbit polyclonal, in-house), and anti-tau (BR134; 1:4000; rabbit polyclonal, in-house). For immunoelectron microscopy, we used the following antibodies: anti-mouse tau (MT1; 1:50; rabbit polyclonal, in-house), BR134 (1:50), AT8 (1:50), and AT100 (1:50).
3
Immuno-profiling of Tauopathy in Tissue
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50 μm‐thick free‐floating sections were incubated in an antigen retrieval solution (Wako, S1700) at 60°C for 30 minutes. After that, sections were incubated in blocking solution: 5% donkey serum, 5% goat serum, 5% bovine serum albumin in PBS 0.3% triton for 2 hours at room temperature under agitation. Sections were then incubated for 48 hours with the following antibodies: IBA‐1 (234‐006, Synaptic Systems, 1:1000), AT8 (MN1020, Invitrogen, 1:500), AT100 (MN1060, Invitrogen, 1:1000), pTau 422 (ab79415, Abcam, 1:1000), NeuN (266004, Syn. Systems, 1:1000), TREM2 (AF1828, R&D systems, 1:500), MAP2 (188004, Syn, Systems, 1:1000), Tau 3R (2A1‐1F4, Wako, 1:400), Tau 4R (ab218314, Abcam, 1:500), CP3, MC1, and PHF1 (provided by Dr. Peter Davies, 1:400). TOC1 antibody for tau oligomers was provided by Dr. Nicholas Kanaan and used at 1:1000. Tissue was washed thoroughly with PBS and incubated with Alexa Fluor secondary antibodies (Invitrogen, 1:500) for 2 hours, at room temperature. True Black solution (Biotium) was used for 30 minutes to eliminate lipofuscin autofluorescence. Slides were then mounted with Prolong Diamond Antifade with Dapi (Invitrogen).
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