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Colorimetric activity assay kit

Manufactured by Beyotime
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Sourced in China

The Colorimetric activity assay kit is a laboratory tool designed to measure the activity of specific enzymes or proteins in a sample. It utilizes a colorimetric method, where the presence or concentration of the target analyte is determined by the intensity of a color change reaction. The core function of this kit is to provide a reliable and quantitative way to assess the activity of the analyte of interest.

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Lab products found in correlation

Microplate reader, by Tecan (1 mentions) Au2700 analyzer, by Olympus (1 mentions)

Close spelling variants of this product

Colorimetric assay kit (35 citations) Colorimetric assay (8 citations) Caspase-3 Colorimetric Activity Assay Kit (7 citations) ALP activity colorimetric assay kit (2 citations)

2 protocols using colorimetric activity assay kit

1

Caspase-3 Activity Colorimetric Assay

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Caspase-3 activity was determined in cytosolic protein extracts using a colorimetric activity assay kit (Beyotime Institute of Biotechnology, Haimen, China) according to the manufacturer's protocol. Specifically, after determining the protein concentration, the supernatant was incubated with the caspase-3 substrate (Ac-DEVD-pNA) on a 96-well-plate. The activity of caspase-3 was detected using a microplate reader (Tecan) at 405 nm.
YAN J., DUAN J., WU X., GUO C., YIN Y., ZHU Y., HU T., WEI G., WEN A, & XI M. (2015). Total saponins from Aralia taibaiensis protect against myocardial ischemia/reperfusion injury through AMPK pathway. International Journal of Molecular Medicine, 36(6), 1538-1546.
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2

Renal Injury Biomarker Evaluation

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Rats were killed 72 h after reperfusion. Blood samples were obtained from the inferior vena cava to measure the concentration of urea nitrogen (BUN) and creatinine (Cr) before surgery. BUN and Cr in blood samples were analyzed using standard techniques with an Olympus AU 2700 Analyzer (Olympus Optical Co., Tokyo, Japan). Levels of MPO and SOD were detected using a standard procedure with a colorimetric activity assay kit (Beyotime, Nanjing, China).
Zhou J.Q., Qiu T., Zhang L., Chen Z.B., Wang Z.S., Ma X.X, & Li D. (2016). Allopurinol preconditioning attenuates renal ischemia/reperfusion injury by inhibiting HMGB1 expression in a rat model. Acta cirurgica brasileira, 31(3).
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