Mosaic digital mirror forphotoactivation

Manufactured by Oxford Instruments

Sourced in Canada

The Mosaic digital mirror for photoactivation is a lab equipment product from Oxford Instruments. It functions as a programmable illumination device for photoactivation experiments.

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Lab products found in correlation

Imageem camera, by Hamamatsu Photonics (2 mentions) Quorum wavefx x1, by Quorum Technologies (2 mentions)

2 protocols using mosaic digital mirror forphotoactivation

Spinning-Disk Confocal Imaging with Photoactivation

Cited 1 time

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Images were acquired using Quorum WaveFX-X1 spinning-disk confocal
system (Quorum Technologies, Canada) equipped with Mosaic digital mirror for
photoactivation (Andor Technology, Connecticut) and Hamamatsu ImageEM camera
(Bridgewater, New Jersey) as previously described (14 (link)).

Bakhoum S.F., Kabeche L., Murnane J.P., Zaki B.I, & Compton D.A. (2014). DNA damage response during mitosis induces whole chromosome mis-segregation. Cancer discovery, 4(11), 1281-1289.

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Measuring Microtubule Turnover Rates

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k-MT attachment stability was assessed by measuring microtubule turnover rates in live U2OS cells stably expressing GFP-tagged α-tubulin (plasmid provided by Alexey Khodjakov). Differential interference contrast (DIC) microscopy was used to identify prometaphase and metaphase mitotic cells. Fluorescent images were acquired using Quorum WaveFX-X1 spinning disc confocal system (Quorum Technologies) equipped with Mosaic digital mirror for photoactivation (Andor Technology) and Hamamatsu ImageEM camera. Prometaphase or metaphase determination was made based on chromosome alignment as visualized by DIC. Microtubules across one half-spindle were laser-illuminated over a defined region, and fluorescence images were subsequently captured every 15 s for a time-course of 4 min with a 100 × oil immersion 1.4 numerical aperture objective.

Dumitru A.M., Rusin S.F., Clark A.E., Kettenbach A.N, & Compton D.A. (2017). Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability. eLife, 6, e29303.

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