hADSCs were purchased from Shenyang Cell Therapy Engineering Technology Research Centre Company. Serum-free cell culture medium (Lonza, USA) supplemented with 10% foetal bovine serum (FBS; Gibco, USA) was used to culture cells at 37°C in a 5% CO2 incubator. In subsequent experiments, hADSCs were divided into the following three groups for culture: CON group (negative control group, which was cultured with Serum-free cell culture medium supplemented with 10% FBS), OM group (positive control group, which was cultured with hADSC OM), and PRP group (experimental group, which was cultured with hADSC OM supplemented with 5% PRP).
Serum free cell culture medium
Manufactured by Lonza
Sourced in United States, Switzerland
Serum-free cell culture medium is a laboratory product designed to support the growth and proliferation of cells in vitro. It is formulated to provide the necessary nutrients and growth factors for cell cultivation without the inclusion of animal-derived serum components.
Automatically generated - may contain errors
2 protocols using serum free cell culture medium
1
Osteogenic Differentiation of hADSCs
2
Hypoxic Expansion of UCB-MNCs
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Isolated UCB-MNC were cultured under 0.5% O2, for 18 h, at a density of 2 million cells/mL in serum-free cell culture medium (Lonza AG, Basel, Switzerland) supplemented with 0.5 μg/mL FMS-like tyrosine kinase-3 (Peprotech, London, UK) and 0.5 μg/mL stem-cell factor (Peprotech, London, UK).
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