Dfc500 12 megapixel camera

Mouse work was approved by the UCSF Institutional Animal Care and Use Committee (IACUC), protocol number AN181381, and was conducted in accordance with AALAC and NIH guidelines. The 12 kb acheiropodia-associated region was amplified from a human BAC (RP11-155D20) by PCR, cloned it into the Hsp68-LacZ vector^{27 (link)} and sequence verified. All LacZ transgenic mice were generated by Cyagen Biosciences using standard procedures⁵⁷ , and harvested and stained for LacZ expression at E11.5 as previously described^{58 (link)}. Pictures were obtained using an M165FC stereo microscope and a DFC500 12-megapixel camera (Leica).

PCR was carried out either on M. lucifugus or M. musculus DNA using primers that were designed to amplify candidate enhancer peak sequences with additional 100–500 bp outside of predicted regions (S1 Table). The bat-mouse BAR116 composite sequence was synthesized (Biomatik) and sequence validated. PCR products and the synthetic sequence were cloned into the Hsp68-LacZ vector [63 (link)] and sequence verified. All transgenic mice were generated by Cyagen Biosciences using standard procedures [88 ], and harvested and stained for LacZ expression at E12.5 as previously described [89 (link)]. Pictures were obtained using an M165FC stereo microscope and a DFC500 12-megapixel camera (Leica). To be designated as an enhancer, we required consistent spatial expression patterns present in at least two embryos.