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Ants 8 aminonaphthalene 1 3 6 trisulfonic acid disodium salt

Manufactured by Thermo Fisher Scientific
Sourced in United States

ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a fluorescent dye used in various analytical techniques. It is a water-soluble compound that exhibits strong fluorescence emission when excited by ultraviolet light. The core function of ANTS is to serve as a labeling agent, enabling the detection and visualization of biomolecules and other analytes in various research and diagnostic applications.

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3 protocols using ants 8 aminonaphthalene 1 3 6 trisulfonic acid disodium salt

1

Lipid Membrane Interaction Assays

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POPE, POPG and palmitoyl-d31 oleoyl phosphatidyglycerol (POPG-d31) were purchased from Avanti Polar Lipids (Alabaster, AL) as powder and used without further purification. L18W-PGLa (GMASKAGAIAGKIAKVAWKAL-NH2), MG2a (GIGKFLHSAKKFGKAFVGEIMNS-NH2), and LF11-215 (FWRIRIRR-NH2) were obtained in lyophilized form (purity > 95%) from PolyPeptide Laboratories (San Diego, CA). ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) and DPX (p-xylene-bis-pyridinium bromide) were purchased from Molecular Probes (Eugene, OR) and HEPES (purity > 99.5%) from Carl Roth (Karlsruhe, Germany). D2O was obtained from Euroisotop (Saarbrücken, Germany), methyl-β-cyclodextrin (mβCD), Triton X-100 and all other chemicals (pro analysis quality) were from Sigma-Aldrich (Vienna, Austria).
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2

Lipid Membrane Characterization Protocol

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The phospholipids 1 palmitoyl-2 oleoyl-sn glycero-3 (phosphor-race-(1 glycerol)) (POPG, PG), 1 palmitoyl-2 oleoyl-sn glycero-3 phosphoethanolamine (POPE, PE), and polar E. coli lipid extract were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Sodium chloride, chloroform, and methanol were obtained from Carl Roth (Graz, Austria) and 4-(2-Hydroxyethyl) piperazine-1 ethanesulfonic acid (HEPES, ≥99.5%) was from Sigma–Aldrich (Vienna, Austria). Unless otherwise indicated, all solutions were prepared in HEPES buffer pH 7.4, containing 10 mM HEPES and 140 mM NaCl or NaPi buffer containing 20 mM NaPi (Na2HPO4/NaH2PO4, 140 mM NaCl, pH = 7.4. ANTS (8-amino naphthalene-1,3,6-trisulfonic acid, disodium salt) and DPX (p-xylene-bis-pyridinium bromide) were from (Molecular Probes, Eugene, OR, USA) and propidium iodide (PI)was from (Invitrogen, Waltham, MA, USA) purchased from Thermo Fischer Scientific (Vienna, Austria).
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3

Liposome Preparation and Characterization

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POPE, POPG, and TOCL were purchased from Avanti Polar Lipids (Alabaster, AL) as powder (purity >99%), and freeze-dried LF11-215 and LF11-324 (purity >95%) were obtained from PolyPeptide Laboratories (San Diego, CA). ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) and DPX (p-xylene-bis-pyridinium bromide) were purchased from Molecular Probes (Eugene, OR) and dimethyl sulfoxide (DMSO) from Sigma-Aldrich (Vienna, Austria). HEPES (purity >99.5%) and LB-agar and LB-medium (Luria/Miller) powders were purchased from Carl Roth (Karlsruhe, Germany). All other chemicals were obtained from Sigma-Aldrich (Vienna, Austria) in pro analysis quality.
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