Ants 8 aminonaphthalene 1 3 6 trisulfonic acid disodium salt

POPE, POPG and palmitoyl-d31 oleoyl phosphatidyglycerol (POPG-d31) were purchased from Avanti Polar Lipids (Alabaster, AL) as powder and used without further purification. L18W-PGLa (GMASKAGAIAGKIAKVAWKAL-NH₂), MG2a (GIGKFLHSAKKFGKAFVGEIMNS-NH₂), and LF11-215 (FWRIRIRR-NH₂) were obtained in lyophilized form (purity > 95%) from PolyPeptide Laboratories (San Diego, CA). ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) and DPX (p-xylene-bis-pyridinium bromide) were purchased from Molecular Probes (Eugene, OR) and HEPES (purity > 99.5%) from Carl Roth (Karlsruhe, Germany). D₂O was obtained from Euroisotop (Saarbrücken, Germany), methyl-β-cyclodextrin (mβCD), Triton X-100 and all other chemicals (pro analysis quality) were from Sigma-Aldrich (Vienna, Austria).

The phospholipids 1 palmitoyl-2 oleoyl-sn glycero-3 (phosphor-race-(1 glycerol)) (POPG, PG), 1 palmitoyl-2 oleoyl-sn glycero-3 phosphoethanolamine (POPE, PE), and polar E. coli lipid extract were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Sodium chloride, chloroform, and methanol were obtained from Carl Roth (Graz, Austria) and 4-(2-Hydroxyethyl) piperazine-1 ethanesulfonic acid (HEPES, ≥99.5%) was from Sigma–Aldrich (Vienna, Austria). Unless otherwise indicated, all solutions were prepared in HEPES buffer pH 7.4, containing 10 mM HEPES and 140 mM NaCl or NaPi buffer containing 20 mM NaPi (Na₂HPO₄/NaH₂PO₄, 140 mM NaCl, pH = 7.4. ANTS (8-amino naphthalene-1,3,6-trisulfonic acid, disodium salt) and DPX (p-xylene-bis-pyridinium bromide) were from (Molecular Probes, Eugene, OR, USA) and propidium iodide (PI)was from (Invitrogen, Waltham, MA, USA) purchased from Thermo Fischer Scientific (Vienna, Austria).

POPE, POPG, and TOCL were purchased from Avanti Polar Lipids (Alabaster, AL) as powder (purity >99%), and freeze-dried LF11-215 and LF11-324 (purity >95%) were obtained from PolyPeptide Laboratories (San Diego, CA). ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) and DPX (p-xylene-bis-pyridinium bromide) were purchased from Molecular Probes (Eugene, OR) and dimethyl sulfoxide (DMSO) from Sigma-Aldrich (Vienna, Austria). HEPES (purity >99.5%) and LB-agar and LB-medium (Luria/Miller) powders were purchased from Carl Roth (Karlsruhe, Germany). All other chemicals were obtained from Sigma-Aldrich (Vienna, Austria) in pro analysis quality.