Eagle 4k ccd

Manufactured by Thermo Fisher Scientific

The Eagle 4k CCD is a high-performance scientific camera designed for a variety of imaging applications. It features a 4,096 x 4,096 pixel CCD sensor, allowing for high-resolution image capture. The camera provides advanced functionality, including flexible exposure control and cooling capabilities, enabling low-noise performance.

Automatically generated - may contain errors

Lab products found in correlation

Tecnai spirit t12, by Thermo Fisher Scientific (2 mentions) Tecnai t20, by Thermo Fisher Scientific (2 mentions) Temcam f416 cmos, by TVIPS (2 mentions) Xplore3d software, by Thermo Fisher Scientific (1 mentions) G2 electron microscope, by Thermo Fisher Scientific (1 mentions) Xplore3d, by Thermo Fisher Scientific (1 mentions) Tecnai g2 spirit biotwin tem, by Thermo Fisher Scientific (1 mentions) G2 20 twin microscope, by Thermo Fisher Scientific (1 mentions) 10 nm colloidal gold particles, by Merck Group (1 mentions)

5 protocols using eagle 4k ccd

Cryo-EM Tomography of Virus Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were mixed with 10 nm gold particles and vitrified as described above. Tomographic tilt-series were recorded in a Tecnai G2 electron microscope operating at 200 kV on a FEI Eagle 4k CCD using the FEI Xplore3D software at a detector magnification of 32,609X (4.6 Å/pixel sampling rate) every 1.5 degrees. Images were acquired with a defocus ranging from 5 to 8 µm, and an accumulated total dose from 90 to 120 e/Å. Tilted series were processed using IMOD [52] (link) and CTF corrected using TOMOCTF [53] (link). A final number of 4 and 3 tomograms were reconstructed for SA11 NTR and TR samples, respectively, using unfiltered weighted back projection algorithms implemented in the TOMO3D package [54] (link) to recover all the high frequencies for subvolume averaging process.

Rodríguez J.M., Chichón F.J., Martín-Forero E., González-Camacho F., Carrascosa J.L., Castón J.R, & Luque D. (2014). New Insights into Rotavirus Entry Machinery: Stabilization of Rotavirus Spike Conformation Is Independent of Trypsin Cleavage. PLoS Pathogens, 10(5), e1004157.

+ Open protocol

+ Expand

Electron Tomography Imaging Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols

For electron tomography, tilt series acquisitions were performed on uranyl acetate and lead citrate-stained 250–300-nm thick resin-embedded sections. Data were obtained between −60° and +60° by using increments of one to two degrees and collected using Xplore3D (FEI, Eindhoven, The Netherlands) on a FEI Tecnai20 200 kV TEM or using Tomography 4.0 (FEI) on a Tecnai G2 Spirit BioTWIN TEM at 120 kV. Images were recorded using an Eagle 4 K CCD (FEI) or a Gatan^® US1000 2 K CCD. Tilt series alignments and back projection reconstructions were performed using eTomo (IMOD) software (University of Colorado, Boulder). Manual contours of features of interest within the tomography reconstructions were drawn using 3dmod (IMOD) software and images were exported to NIH Image J (v1.48r) for the generation of model animations.

Davis L.J., Bright N.A., Edgar J.R., Parkinson M.D., Wartosch L., Mantell J., Peden A.A, & Luzio J.P. (2021). Organelle tethering, pore formation and SNARE compensation in the late endocytic pathway. Journal of Cell Science, 134(10), jcs255463.

+ Open protocol

+ Expand

Immunogold Labeling of Callose in Leaf Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spurr's resin blocks containing the sectioned leaves with preserved polysaccharide antigenicity and fine structure were immunogold labeled for callose. Preloaded nickel grids carrying ultrathin leaf sections or cell wall or PD fractions were incubated with the monoclonal anti-b-(1,3)-glucan antibody (1: 20 dilution, Biosupplies) or the PDLP5 polyclonal antiserum (1: 20 dilution) at room temperature for 1 h and then at 4 C overnight. Goat anti-mouse or anti-rabbit immunoglobulin G antibody conjugated with 10-nm colloidal gold particles (1:50 dilution, Sigma-Aldrich) was used as the secondary antibody. Before TEM (Tecnai G2 20 TWIN microscope equipped with an Eagle 4k CCD at 120 kV, FEI), the sections were stained with 2% (w/v) uranyl acetate. With regard to the immunogold labeling of callose, both branched and simple PDs of mesophyll cells from 4-week-old functional mature leaf tissues were scored. According to the general definition, these leaves were source leaves.

Liu N.J., Zhang T., Liu Z.H., Chen X., Guo H.S., Ju B.H., Zhang Y.Y., Li G.Z., Zhou Q.H., Qin Y.M, & Zhu Y.X. (2020). Phytosphinganine Affects Plasmodesmata Permeability via Facilitating PDLP5-Stimulated Callose Accumulation in Arabidopsis. Molecular plant, 13(1).

+ Open protocol

+ Expand

Structural Analysis of Fab/IgG-SOSIP Complexes

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fab/IgG-SOSIP complexes were made by incubating Fabs or IgGs with AMC009 SOSIP trimers with a 4-fold and 2-fold molar excess of antibody, respectively, for 30 minutes at RT. The Fab/IgG-SOSIP complexes were loaded onto glow-discharged, carbon-coated Cu400 EM grids at a concentration of 30 ng/μl in TBS for 10 seconds. The grids were blotted to remove excess sample and the Fab/IgG-SOSIP complexes were then stained with 2% (w/v) uranyl formate. Grids were immediately blotted and a second stain with 2% (w/v) uranyl formate was applied for 30 seconds, followed by a final blot to remove excess stain. A Tecnai Spirit T12 (FEI) (120kV, 52,000x magnification) equipped with an Eagle 4K CCD (FEI/Thermo Fisher) or Tecnai T20 (FEI) (200kV, 62,000x magnification) equipped with a TemCam F416 CMOS (TVIPS)was used to image the grids. Image collection was performed using Leginon and data processing was carried out as previously described [60 (link),61 (link)]. 2D classification and 3D sorting was performed with Relion v3.0 [62 (link)], and UCSF Chimera [63 (link)] and Segger [64 (link)] were used to visualize and segment the EM maps, respectively.

van Schooten J., Schorcht A., Farokhi E., Umotoy J.C., Gao H., van den Kerkhof T.L., Dorning J., Rijkhold Meesters T.G., van der Woude P., Burger J.A., Bijl T., Ghalaiyini R., Torrents de la Peña A., Turner H.L., Labranche C.C., Stanfield R.L., Sok D., Schuitemaker H., Montefiori D.C., Burton D.R., Ozorowski G., Seaman M.S., Wilson I.A., Sanders R.W., Ward A.B, & van Gils M.J. (2022). Complementary antibody lineages achieve neutralization breadth in an HIV-1 infected elite neutralizer. PLOS Pathogens, 18(11), e1010945.

+ Open protocol

+ Expand

Cryo-EM Analysis of Fab/IgG-SOSIP Complexes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fabs or IgGs were complexed with SOSIP trimers for 30 min at RT with a 4-fold and 2-fold molar excess of Fab and IgG, respectively. Fab/IgG-SOSIP complexes were diluted to 30 ng/µl in TBS and loaded onto glow-discharged, carbon-coated Cu400 EM grids for 10 s followed by blotting to remove excess sample. The Fab/IgG-SOSIP complexes were then stained with 2% (w/v) uranyl formate and immediately blotted, followed by a second stain with 2% (w/v) uranyl formate for 30 s before blotting to remove excess stain. Image collection was performed on a Tecnai Spirit T12 (FEI) (120 kV, ×52,000 magnification) equipped with an Eagle 4 K CCD (FEI/Thermo Fisher) camera or Tecnai T20 (FEI) (200 kV, ×62,000 magnification) equipped with a TemCam F416 CMOS (TVIPS) camera using Leginon. Data processing was performed as follows^{57 (link),58 (link)}. 2D classification and 3D sorting was performed using Relion v3.0⁵⁹ and EM maps were visualized and segmented using UCSF Chimera^{60 (link)} and Segger^{61 (link)}, respectively.

van Schooten J., Farokhi E., Schorcht A., van den Kerkhof T.L., Gao H., van der Woude P., Burger J.A., Meesters T.G., Bijl T., Ghalaiyini R., Turner H.L., Dorning J., van Schaik B.D., van Kampen A.H., Labranche C.C., Stanfield R.L., Sok D., Montefiori D.C., Burton D.R., Seaman M.S., Ozorowski G., Wilson I.A., Sanders R.W., Ward A.B, & van Gils M.J. (2022). Identification of IOMA-class neutralizing antibodies targeting the CD4-binding site on the HIV-1 envelope glycoprotein. Nature Communications, 13, 4515.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!