Spectra por 3 rc dialysis membrane

Simvastatin, ethylenediamine, methyl acrylate, succinic anhydride, α-D-glucoheptono-1,4-lactone (GHL), carbonyl diimidazole (CDI), 2-chloro-1-methylpyridinium iodide, 4-dimethylaminopyridine (DMAP), triethylamine (TEA), R-glycidol, dimethyl sulfoxide (DMSO), and other reagents used in syntheses were obtained from Merck KGaA (Darmstadt, Germany). Celecoxib was purchased from Abcam GmbH (Karlsruhe, Germany), while biotin N-hydroxysuccinimide ester (NHS-Biotin) was obtained from AmBeed (Arlington Hts, IL, USA). Spectra/Por^® 3 RC dialysis membrane (cellulose, MW_cutoff—3.5 kD) was provided by Carl Roth GmbH and Co. KG (Karlsruhe, Germany).

All the chemicals used during the synthesis of PAMAM G4 dendrimer and its conjugates were purchased from Merck KGaA (Darmstadt, Germany). PAMAM G4 dendrimer was obtained at a 2 millimolar scale according to the procedure published by Tomalia et al. [103 (link)] and stored as a 15 mM solution in methanol to obtain conjugates with fulvestrant, paclitaxel, and lapatinib (AmBeed, Arlington Hts, IL 60004, USA). Further, 4-nitrophenyl chloroformate (NPCF), 2-fluorescein isothiocyanate (FITC), 4-dimethylaminopyridin (DMAP), and 1-methyl-2-chloropyridinium iodide (pyI) were purchased from Merck KGaA (Darmstadt, Germany). Spectra/Por^® 3 RC dialysis membrane (cellulose, MW_cutoff—3.5 kDa) was provided by Carl Roth GmbH & Co., KG (Karlsruhe, Germany).