Hdg9623

Manufactured by Baxter

The HDG9623 is a compact and versatile laboratory equipment designed for general laboratory use. It serves as a reliable tool for various scientific applications. The key function of this product is to provide a consistent and controlled environment for laboratory processes, but a more detailed description is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

Bf120 69 10, by Sutter Instruments (2 mentions) F7252, by Merck Group (1 mentions) Fast green, by Merck Group (1 mentions)

3 protocols using hdg9623

In utero Electroporation of Murine Embryos

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Wild‐type C57BL/6J pregnant mice carrying E13.5 embryos were anesthetized using initially 4% isoflurane (Baxter, HDG9623), followed by 2–3% isoflurane during the in utero electroporation (IUE) procedure. The animals were injected subcutaneously with the analgesic (0.1 ml of metamizol, 200 mg/kg). The peritoneal cavity was surgically opened and the uterus exposed. Using borosilicate microcapillary (Sutter instruments, BF120‐69‐10), the embryos were injected intraventricularly with a solution containing 0.1% Fast green (Sigma‐Aldrich, F7252) in sterile PBS, 2 µg/µl of pSuper plasmid (either 2 µg/µl of pSuper‐shcon or 1 µg/µl of pSuper‐shCcny and 1 µg/µl of pSuper‐shCcnyl1), 0.4 µg/µl of pCAGGS GFP. The electroporations (six 50‐msec pulses of 28V at 1 s intervals) were performed using a 3‐mm diameter electrode (BTX genetronics Inc., 45‐0052INT). After surgery, mice received Metamizol in drinking water (1.33 mg/ml).
Pregnant mice were sacrificed by cervical dislocation at the indicated time points (E15.5‐E17.5), and embryonic brains were dissected, fixed in 4% PFA, overnight at 4°C and processed for cryo‐sectioning.

Da Silva F., Zhang K., Pinson A., Fatti E., Wilsch‐Bräuninger M., Herbst J., Vidal V., Schedl A., Huttner W.B, & Niehrs C. (2021). Mitotic WNT signalling orchestrates neurogenesis in the developing neocortex. The EMBO Journal, 40(19), e108041.

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In Utero Electroporation of Murine Embryos

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Tamoxifen-treated pregnant mice carrying E13.5 embryos were anesthetized using initially 5% isoflurane (Baxter, HDG9623), followed by 2%–3% isoflurane during the IUE procedure. Endotoxin-free plasmids (Control, pCAGGS–LoxP–Gap43-GFP–LoxP–IRES–nRFP; CA-YAP, pCAGGS–LoxP–Gap43-GFP–LoxP–CA-YAP–IRES–nRFP) were mixed on the day of surgery with Fast Green (Sigma, 0.25% final concentration) to a final plasmid concentration of 2 μg/μl in 1x PBS. Using a borosilicate microcapillary (Sutter instruments, BF120-69-10) the DNA/Fast Green mixture was intraventricularly injected, which was followed by six 50-msec pulses of 30 V at 1 s intervals (BTX genetronics Inc., 45-0052INT), using a 3-mm diameter electrode (BTX genetronics Inc., 45-0487). After the IUE, the uterus was placed back into the abdominal cavity, and the peritoneum was sutured (VICRYL 5-0, V493H). Abdominal skin was closed with clips and animal received 100 μl of painkiller (Rimadyl 1 mg/ml). Pregnant mice were sacrificed by cervical dislocation at the indicated time points (E14.5-E17.5), and embryonic brains were dissected and fixed in 4% PFA, overnight at 4°C.

Kostic M., Paridaen J.T., Long K.R., Kalebic N., Langen B., Grübling N., Wimberger P., Kawasaki H., Namba T, & Huttner W.B. (2019). YAP Activity Is Necessary and Sufficient for Basal Progenitor Abundance and Proliferation in the Developing Neocortex. Cell Reports, 27(4), 1103-1118.e6.

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Stereotaxic Brain Injury Protocol

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The brain tissue injury was induced by stereotaxic injection. The mouse was anesthetized with a combination of oxygen and isoflurane (49:1) (Baxter–HDG9623) flow during the procedure and put on a pre-warmed heat-pad to avoid hypothermia. Ear bars were used to keep the head immobile, and a protective ointment was applied to the eyes to prevent cornea dehydration. An analgesic was administered subcutaneously before to the surgery to reduce any potential pain afterward. The injury was caused in the right hemisphere, coordinates were ± 1.6 mm mediolateral, − 1.9 mm anterior–posterior, and − 1.9 mm dorsoventral from the Bregma, where the PBS was dispensed at 200 nL/min speed. The left hemisphere was used as a control for the analysis. The capillary was progressively retracted after the injection, followed by the ear being released. Mouse brain was analyzed 3 days after the injury via immunohistochemistry.

Lee A.J., Raghavan N.S., Bhattarai P., Siddiqui T., Sariya S., Reyes-Dumeyer D., Flowers X.E., Cardoso S.A., De Jager P.L., Bennett D.A., Schneider J.A., Menon V., Wang Y., Lantigua R.A., Medrano M., Rivera D., Jiménez-Velázquez I.Z., Kukull W.A., Brickman A.M., Manly J.J., Tosto G., Kizil C., Vardarajan B.N, & Mayeux R. (2022). FMNL2 regulates gliovascular interactions and is associated with vascular risk factors and cerebrovascular pathology in Alzheimer’s disease. Acta Neuropathologica, 144(1), 59-79.

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