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L15 phenol red free media

Manufactured by Thermo Fisher Scientific
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L15 phenol-red free media is a cell culture medium formulated without the use of phenol red indicator. It is commonly used in the maintenance and growth of various cell lines.

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Lab products found in correlation

Fluorescent gelatin matrix, by Thermo Fisher Scientific (2 mentions) Lsm 880 microscope, by Zeiss (2 mentions)

Close spelling variants of this product

L15 phenol red free cell culture media Phenol red-free media Phenol red Phenol

2 protocols using l15 phenol red free media

1

Visualizing Cell Motility in Fluorescent Matrix

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated on either 1 mg/mL fluorescent gelatin matrix (Thermo Fisher) or fibrillar collagen I for 2–4 h and then imaged in L15 phenol-red free media (Gibco) with 10% FBS (Gemini Bio) using an inverted confocal Zeiss LSM 880 microscope. Imaging was conducted in a 37°C humidified and temperature-controlled chamber. Z-stacks were acquired every 30 min for 24 cycles with a 63X 1.4 NA objective, with a z-step size of 0.8–1 μm. Analysis of cell motility parameters was done with Imaris (Bitplane, Switzerland).
Mondal C., Gacha-Garay M.J., Larkin K.A., Adikes R.C., Di Martino J.S., Chien C.C., Fraser M., Eni-aganga I., Agullo-Pascual E., Cialowicz K., Ozbek U., Naba A., Gaitas A., Fu T.M., Upadhyayula S., Betzig E., Matus D.Q., Martin B.L, & Bravo-Cordero J.J. (2022). A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling. Cell reports, 40(12), 111358.
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2

Visualizing Cell Motility in Fluorescent Matrix

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated on either 1 mg/mL fluorescent gelatin matrix (Thermo Fisher) or fibrillar collagen I for 2–4 h and then imaged in L15 phenol-red free media (Gibco) with 10% FBS (Gemini Bio) using an inverted confocal Zeiss LSM 880 microscope. Imaging was conducted in a 37°C humidified and temperature-controlled chamber. Z-stacks were acquired every 30 min for 24 cycles with a 63X 1.4 NA objective, with a z-step size of 0.8–1 μm. Analysis of cell motility parameters was done with Imaris (Bitplane, Switzerland).
Mondal C., Gacha-Garay M.J., Larkin K.A., Adikes R.C., Di Martino J.S., Chien C.C., Fraser M., Eni-aganga I., Agullo-Pascual E., Cialowicz K., Ozbek U., Naba A., Gaitas A., Fu T.M., Upadhyayula S., Betzig E., Matus D.Q., Martin B.L, & Bravo-Cordero J.J. (2022). A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling. Cell reports, 40(12), 111358.
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