Double sided carbon sticky tabs

Manufactured by Agar Scientific

Sourced in United Kingdom

Double-sided carbon sticky tabs are a laboratory consumable item used for the mounting and securing of samples or other materials in various scientific applications. These tabs provide a reliable and adhesive surface on both sides, allowing for the attachment of specimens or components to substrates or equipment.

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Lab products found in correlation

Xl30 feg sem, by Philips (1 mentions) Sem stub, by Agar Scientific (1 mentions) Leo 1550 fe sem, by Zeiss (1 mentions)

2 protocols using double sided carbon sticky tabs

Evaluating Collagen Alignment in EngNT Preservation

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Scanning electron microscopy (SEM) was used to assess whether EngNT preserved in Hibernate-A or 60% DMEM, 30% FBS, and 10% DMSO for 3 days affected collagen alignment compared with EngNT maintained in normal culture conditions for 3 days (no preservation). Samples were fixed in 3% glutaraldehyde for at least 24 h at 4°C, then dehydrated through an increasing graded series of ethanol. Finally, samples were fully immersed in hexamethyldisilazane for 2 min, which was then removed, and samples were left to dry. Samples were mounted onto aluminum SEM stubs using double-sided carbon sticky tabs (Agar Scientific, United Kingdom), sputter coated with gold palladium, and imaged using a Philips XL30 FEGSEM at 5 kV.

Day A.G., Bhangra K.S., Murray-Dunning C., Stevanato L, & Phillips J.B. (2017). The Effect of Hypothermic and Cryogenic Preservation on Engineered Neural Tissue. Tissue Engineering. Part C, Methods, 23(10), 575-582.

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Scanning Electron Microscopy of Samples

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Samples were removed from culture, washed with PBS and fixed using 2.5% (w/v) glutaraldehyde for 15 min at room temperature, washed with PBS and submerged in 70% ethanol for 15 min, then 90% ethanol for 15 min followed by two changes of 100% ethanol for 15 min. Samples were dried using a critical point dryer (Prion, UK). Dry samples were fixed to SEM stubs (Agar Scientific, UK) using double-sided carbon sticky tabs (Agar Scientific, UK). The samples were then coated with 20 nm of chromium using a sputter coater (Emtech, UK). The samples were observed under a Leo 1550 FESEM (Zeiss, UK). This was repeated 3 times for each time point. Representative images were used in results section. Where nodule formation was observed, measurements were taken using the annotation function on the LEO SEM software (Zeiss, Germany).

Fawcett S.A., Curran J.M., Chen R., Rhodes N.P., Murphy M.F., Wilson P., Ranganath L., Dillon J.P., Gallagher J.A, & Hunt J.A. (2016). Defining the Properties of an Array of –NH2-Modified Substrates for the Induction of a Mature Osteoblast/Osteocyte Phenotype from a Primary Human Osteoblast Population Using Controlled Nanotopography and Surface Chemistry. Calcified Tissue International, 100(1), 95-106.

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