Enhanced chemiluminescence ecl western blot detection reagent

RPMI1640, fetal bovine serum (FBS) and the enhanced chemiluminescence (ECL) Western blot detection reagent were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, United States). Mouse anti-dinitrophenyl (DNP) IgE was purchased from Sigma Chemicals (St. Louis, MO, United States). DNP-human serum albumin (HSA) was from Biosearch Technologies (Petaluma, CA, United States). The antibodies specific for phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-JNK1/2, JNK1/2, phospho-PLCγ, phospho-IκBα, IκBα, phospho-IKKα/β, β-actin, and the horseradish peroxidase-conjugated goat anti-rabbit secondary antibody were purchased from Cell Signaling Technology, Inc. (Danvers, MA, United States). The antibodies specific for phospho-cPLA₂, NF-κB p65, lamin B, LAT, Lyn, Fyn, and Syk, as well as Bay 61-3606 reagent were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, United States). The LTC₄ enzyme linked immunoassay (EIA) kit, and the antibody for COX-2 were from Cayman Chemical (Ann Arbor, MI, United States). Histamine ELISA kit was purchased from Demeditec Diagnostics GmbH (Kiel, Germany).

RPMI1640, fetal bovine serum (FBS), and the enhanced chemiluminescence (ECL) Western blot detection reagent were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Mouse anti-dinitrophenyl (DNP) IgE was purchased from Sigma Chemicals (St. Louis, MO, USA). DNP-HSA was from Biosearch Technologies (Petaluma, CA, USA). The antibodies specific for phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-PLCγ, phospho-IκB, IκB, phospho-IKKα/β, β-actin, and the horseradish peroxidase-conjugated goat anti-rabbit secondary antibody were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The antibodies specific for phospho-cPLA₂, NF-κB p65, lamin B, Lyn, Fyn, and Syk, as well as Bay 61-3606 reagent were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The LTC₄ enzyme linked immunoassay (EIA) kit, and the antibody for COX-2 were from Cayman Chemical (Ann Arbor, MI, USA). Histamine enzyme linked immunosorbent assay (ELISA) kit was purchased from Demeditec Diagnostics GmbH (Kiel, Germany). AB23A was obtained from Push Bio-technology Co., Ltd (Chengdu, China), of which the purity is ≥98%.