Anti pi3 kinase p110β

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-PI3 kinase p110β is a laboratory research antibody that targets the p110β subunit of phosphoinositide 3-kinase (PI3K). PI3K is an enzyme complex that plays a key role in various cellular processes.

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2 protocols using anti pi3 kinase p110β

Western Blot Analysis of Glycolytic Enzymes

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Preparation of total cell lysates and Western blot analysis were performed as described previously [33 (link)]. A total of 10–50 µg of protein was used per lane. The blot was probed with anti-GLUT1 (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-HK2 (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-PKM2 (1:1000, Cell Signaling), anti-LDHA (1:1000, Cell Signaling), anti-p85α (1:1000, Cell Signaling), anti-PI3 kinase p110α (1:1000, Cell Signaling), anti-PI3 kinase p110β (1:1000, Cell Signaling), anti-phospho-AKT (Ser473) (1:1000, Cell Signaling), anti-AKT (1:1000, Cell Signaling), anti-PDK1 (1:1000, Cell Signaling), anti-FOXO3a (1:1000, Cell Signaling), and anti-cMYC (1:1000, Cell Signaling) antibodies. The relative densities of bands were analyzed with NIH image J 1.47v software.

Kim S., Lee E., Jung J., Lee J.W., Kim H.J., Kim J., Yoo H.J., Lee H.J., Chae S.Y., Jeon S.M., Son B.H., Gong G., Sharan S.K, & Chang S. (2018). microRNA-155 positively regulates glucose metabolism via PIK3R1-FOXO3a-cMYC axis in breast cancer. Oncogene, 37(22), 2982-2991.

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Antibody Use for Cellular Protein Analysis

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Antibodies used in this study are as follows. Anti-human Claspin, anti-mouse Claspin, anti-MCM4, and anti-MCM4 S6T7 were previously described.¹⁹ (link) Anti-LaminB (sc-6216), anti-Chk1 (sc-8408), anti-p53 (sc-99), anti-Claspin (sc-376773) and anti-MCM2 (sc-9839) were from Santa Cruz Biotechnology; anti-Flag (M185-3L) were from MBL; anti-Chk1 S345(#2341 or #2348), anti-Chk1 S317 (#2344), anti-mTor (#2983), anti-mTOR S2448 (#5536), anti-p44/42 MAPK (Erk1/2) (#4695), anti- SAPK/JNK (#9252), p38 MAPK (#8690), anti-p38 MAPK T180/Y182 (#4511), anti-p44/42 MAPK (Erk1/2) T202/Y204 (#4370), anti-SAPK/JNK T183/Y185 (#4668), anti-PDK1 (#3062), anti-PDK1 S241 (#3438), anti-PI3 kinase p110β (#3011), anti-PI3 kinase p110γ (#5405), anti-PI3 kinase p110α (#4249), anti-PI3 kinase class III (#3358), anti- p70 S6 kinase T389 (#9234), anti-p70 S6 kinase (#9202), anti-4E-BP1 T37/T46 (#2855), anti-4E-BP1 (#9644), anti-Caspase-3 (#14220), anti-Akt S473(#4058) and anti-Akt (#4691) were from Cell signaling; anti-tubulin (T5168) was from Sigma-Aldrich; anti-MCM2 S53(A300-756A) was from Bethyl; anti-p53 S37 (PAB12641) was from Abnova; anti-histone H2A.X Ser139 (05-636) and anti-histone H3 Ser10 (05-598) were from Merck Millipore; anti-Rb (GTX50459) was from GeneTex.

Yang C.C, & Masai H. (2023). Claspin is Required for Growth Recovery from Serum Starvation through Regulating the PI3K-PDK1-mTOR Pathway in Mammalian Cells. Molecular and Cellular Biology, 43(1), 1-21.

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