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4 protocols using tryptone

1

Antioxidant Assays Protocol

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Agar powder, tryptone, 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), glutathione and 2′,7′-Dichlorofluorescein were purchased from Solarbio Co. (Beijing, China). Cholesterol, paraquat and Vitamin C (Vc) were purchased from Sigma-Aldrich (St. Louis, MO). Methanol was purchased from Merck KGaA (Germany). Biowest Agarose was purchased from wobisen technology co. (Beijing, China). All the other chemicals were standard commercial products of analytical-reagent grade.
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2

Biopolymer-Based Microcapsule Synthesis

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Petroleum ether (PE), sodium
carbonate (Na2CO3), anhydrous ethanol, sodium
chloride (NaCl), and isopropyl alcohol were obtained from Sinopharm.
Lithium bromide (LiBr) and Span-80 were purchased from Aladdin. Branched
polyethylenimine (PEI, Mw ∼ 25,000) was acquired from Sigma-Aldrich.
Polyethylene glycol 8000 (PEG-8000), vancomycin, yeast extract, tryptone,
agar power, DNase I, phosphate-buffered saline (PBS), penicillin/streptomycin,
and a CCK-8 assay kit (CA1210) were purchased from Solarbio. A live/dead
staining kit (L34951) was obtained from Thermo Fisher Scientific.
FITC and rhodamine B were purchased from Macklin.
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3

Fabrication of Antimicrobial Curcumin Nanoparticles

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Silver nitrate, sodium chloride, and N, N-dimethylformamide (DMF) were purchased from Sinopharm Chemical Reagent Co., Ltd. Curcumin (Cur), polycaprolactone (PCL, Mw = 8×104), and polyvinylpyrrolidone K-90 (PVP, Mw = 1.3×106) were obtained from Dalian Meilun Biological Co., Ltd. Chloroform (CHCl3) was bought from Laiyang Kangde Chemical Co., Ltd., and ethanol (C2H5OH) was purchased from Tianjin Fuyu Fine Chemical Co., Ltd. Beef extract was obtained from Beijing Obosing Biotechnology Company. Tryptone was bought from OXOID, and agar powder was purchased from Solarbio. Staphylococcus aureus and Escherichia coli were obtained from Beijing Beina Chuanglian Institute of Biotechnology. Methicillin-resistant Staphylococcus aureus was purchased from Shanghai Biotechnology Center, and 2, 2, 6, 6-Tetramethylpiperidine (TEMP) was bought from Tongren Institute of Chemistry.
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4

Microbial Growth Cultivation Protocol

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Yeast powder, agar powder, tryptone, and glycerol were obtained from Solarbio Technology Co., Ltd., Beijing, China. The E. coli ATCC 25312 strain and S. enteritidis ATCC 29629 strain were obtained from Biotech Co., Ltd., Beijing, China. All chemicals were of analytical grade. The distilled water and chemicals involved in the study were used under sterile conditions.
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